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@ARTICLE{Oeggl:902210,
      author       = {Oeggl, Reinhard and Glaser, Juliane and von Lieres, Eric
                      and Rother, Dörte},
      title        = {{C}ontinuous enzymatic stirred tank reactor cascade with
                      unconventional medium yielding high concentrations of
                      ({S})-2-hydroxyphenyl propanone and its derivatives},
      journal      = {Catalysis science $\&$ technology},
      volume       = {11},
      number       = {24},
      issn         = {2044-4761},
      address      = {London},
      publisher    = {RSC Publ.},
      reportid     = {FZJ-2021-04098},
      pages        = {7886-7897},
      year         = {2021},
      abstract     = {The implementation of biocatalysis in flow chemistry offers
                      synergistic synthesis advantages in line with green
                      chemistry principles. Yet, the conversion of high substrate
                      concentrations is in many cases hindered by insolubility
                      issues or substrate toxicity. Here, the continuous synthesis
                      of (S)-2-hydroxyphenyl propanone (2-HPP) from inexpensive
                      benzaldehyde and acetaldehyde in a methyl tert-butyl ether
                      based organic reaction environment, namely micro-aqueous
                      reaction system, has been established. Kinetic parameters of
                      the applied whole cell catalyst were identified to design a
                      continuous process for (S)-2-HPP synthesis. This revealed a
                      necessity to distribute acetaldehyde over a spatial
                      coordinate to remain below a toxic concentration threshold.
                      Hence, three continuous stirred tank reactors (cSTR) were
                      conjugated in a technical cascade with an additional influx
                      of acetaldehyde into each unit. The catalytic behaviour of
                      this reaction setup was described based on mass balances and
                      a kinetic model. Enzyme deactivation was described by a
                      novel staged model and compared to a simple generic model.
                      The optimized continuous setup yielded 190 mM (S)-HPP with
                      an ee > $98\%$ over 8 h. The product was easily recovered
                      from the organic reaction environment by crystallization
                      with an isolated yield of $68\%$ and a purity of $>99\%.$
                      Further, the substrate range of the applied catalyst
                      Pseudomonas putida benzoylformate decarboxylase variant
                      L461A was analysed. This revealed numerous halogenated,
                      methoxylated and nitro-derivatives in ortho, meta, and para
                      position, which can in principle be gained by the
                      established process. As an example, the applied cSTR concept
                      was transferred to p-methoxy benzaldehyde with good results
                      even without further optimization.},
      cin          = {IBG-1},
      ddc          = {540},
      cid          = {I:(DE-Juel1)IBG-1-20101118},
      pnm          = {2172 - Utilization of renewable carbon and energy sources
                      and engineering of ecosystem functions (POF4-217)},
      pid          = {G:(DE-HGF)POF4-2172},
      typ          = {PUB:(DE-HGF)16},
      UT           = {WOS:000714119900001},
      doi          = {10.1039/D0CY01666G},
      url          = {https://juser.fz-juelich.de/record/902210},
}