TY  - JOUR
AU  - Ryzhykau, Yury L.
AU  - Orekhov, Philipp S.
AU  - Rulev, Maksim I.
AU  - Vlasov, Alexey V.
AU  - Melnikov, Igor A.
AU  - Volkov, Dmytro A.
AU  - Nikolaev, Mikhail Yu.
AU  - Zabelskii, Dmitrii V.
AU  - Murugova, Tatiana N.
AU  - Chupin, Vladimir V.
AU  - Rogachev, Andrey V.
AU  - Gruzinov, Andrey Yu.
AU  - Svergun, Dmitri I.
AU  - Brennich, Martha E.
AU  - Gushchin, Ivan Yu.
AU  - Soler-Lopez, Montserrat
AU  - Bothe, Arne
AU  - Büldt, Georg
AU  - Leonard, Gordon
AU  - Engelhard, Martin
AU  - Kuklin, Alexander I.
AU  - Gordeliy, Valentin I.
TI  - Molecular model of a sensor of two-component signaling system
JO  - Scientific reports
VL  - 11
IS  - 1
SN  - 2045-2322
CY  - [London]
PB  - Macmillan Publishers Limited, part of Springer Nature
M1  - FZJ-2021-04118
SP  - 10774
PY  - 2021
AB  - Two-component systems (TCS) are widespread signaling systems present in all domains of life. TCS typically consist of a signal receptor/transducer and a response regulator. The receptors (histidine kinases, chemoreceptors and photoreceptors) are often embedded in the membrane and have a similar modular structure. Chemoreceptors were shown to function in highly ordered arrays, with trimers of dimers being the smallest functional unit. However, much less is known about photoreceptors. Here, we use small-angle scattering (SAS) to show that detergent-solubilized sensory rhodopsin II in complex with its cognate transducer forms dimers at low salt concentration, which associate into trimers of dimers at higher buffer molarities. We then fit an atomistic model of the whole complex into the SAS data. The obtained results suggest that the trimer of dimers is "tripod"-shaped and that the contacts between the dimers occur only through their cytoplasmic regions, whereas the transmembrane regions remain unconnected.
LB  - PUB:(DE-HGF)16
C6  - pmid:34031444
UR  - <Go to ISI:>//WOS:000659113400019
DO  - DOI:10.1038/s41598-021-89613-6
UR  - https://juser.fz-juelich.de/record/902248
ER  -