% IMPORTANT: The following is UTF-8 encoded.  This means that in the presence
% of non-ASCII characters, it will not work with BibTeX 0.99 or older.
% Instead, you should use an up-to-date BibTeX implementation like “bibtex8” or
% “biber”.

@ARTICLE{Rosenbach:902421,
      author       = {Rosenbach, Hannah and Borggräfe, Jan and Victor, Julian
                      and Wuebben, Christine and Schiemann, Olav and Hoyer,
                      Wolfgang and Steger, Gerhard and Etzkorn, Manuel and Span,
                      Ingrid},
      title        = {{I}nfluence of monovalent metal ions on metal binding and
                      catalytic activity of the 10–23 {DNA}zyme},
      journal      = {Biological chemistry},
      volume       = {402},
      number       = {1},
      issn         = {1431-6730},
      address      = {Berlin [u.a.]},
      publisher    = {de Gruyter},
      reportid     = {FZJ-2021-04243},
      pages        = {99 - 111},
      year         = {2020},
      abstract     = {Deoxyribozymes (DNAzymes) are single-stranded DNA molecules
                      that catalyze a broad range of chemical reactions. The
                      10–23 DNAzyme catalyzes the cleavage of RNA strands and
                      can be designed to cleave essentially any target RNA, which
                      makes it particularly interesting for therapeutic and
                      biosensing applications. The activity of this DNAzyme in
                      vitro is considerably higher than in cells, which was
                      suggested to be a result of the low intracellular
                      concentration of bioavailable divalent cations. While the
                      interaction of the 10–23 DNAzyme with divalent metal ions
                      was studied extensively, the influence of monovalent metal
                      ions on its activity remains poorly understood. Here, we
                      characterize the influence of monovalent and divalent
                      cations on the 10–23 DNAzyme utilizing functional and
                      biophysical techniques. Our results show that Na+ and K+
                      affect the binding of divalent metal ions to the DNAzyme:RNA
                      complex and considerably modulate the reaction rates of RNA
                      cleavage. We observe an opposite effect of high levels of
                      Na+ and K+ concentrations on Mg2+- and Mn2+-induced
                      reactions, revealing a different interplay of these metals
                      in catalysis. Based on these findings, we propose a model
                      for the interaction of metal ions with the DNAzyme:RNA
                      complex.},
      cin          = {IBI-7},
      ddc          = {570},
      cid          = {I:(DE-Juel1)IBI-7-20200312},
      pnm          = {552 - Engineering Cell Function (POF3-552)},
      pid          = {G:(DE-HGF)POF3-552},
      typ          = {PUB:(DE-HGF)16},
      UT           = {WOS:000596049300010},
      doi          = {10.1515/hsz-2020-0207},
      url          = {https://juser.fz-juelich.de/record/902421},
}