000902465 001__ 902465
000902465 005__ 20211130111056.0
000902465 0247_ $$2doi$$a10.1016/j.chroma.2021.462412
000902465 0247_ $$2ISSN$$a0021-9673
000902465 0247_ $$2ISSN$$a0376-737x
000902465 0247_ $$2ISSN$$a0378-4355
000902465 0247_ $$2Handle$$a2128/29028
000902465 0247_ $$2altmetric$$aaltmetric:109655201
000902465 0247_ $$2pmid$$apmid:34320430
000902465 0247_ $$2WOS$$aWOS:000677692300009
000902465 037__ $$aFZJ-2021-04284
000902465 082__ $$a540
000902465 1001_ $$00000-0002-4160-7760$$aBeck, Jürgen$$b0
000902465 245__ $$aPatterns of protein adsorption in ion-exchange particles and columns: Evolution of protein concentration profiles during load, hold, and wash steps predicted for pore and solid diffusion mechanisms
000902465 260__ $$aNew York, NY [u.a.]$$bScience Direct$$c2021
000902465 3367_ $$2DRIVER$$aarticle
000902465 3367_ $$2DataCite$$aOutput Types/Journal article
000902465 3367_ $$0PUB:(DE-HGF)16$$2PUB:(DE-HGF)$$aJournal Article$$bjournal$$mjournal$$s1637062674_17048
000902465 3367_ $$2BibTeX$$aARTICLE
000902465 3367_ $$2ORCID$$aJOURNAL_ARTICLE
000902465 3367_ $$00$$2EndNote$$aJournal Article
000902465 520__ $$aElucidation of protein transport mechanism in ion exchanges is essential to model separation performance. In this work we simulate intraparticle adsorption profiles during batch adsorption assuming typical process conditions for pore, solid and parallel diffusion. Artificial confocal laser scanning microscopy images are created to identify apparent differences between the different transport mechanisms. Typical sharp fronts for pore diffusion are characteristic for Langmuir equilibrium constants of KL ≥1. Only at KL = 0.1 and lower, the profiles are smooth and practically indistinguishable from a solid diffusion mechanism. During hold and wash steps, at which the interstitial buffer is removed or exchanged, continuation of diffusion of protein molecules is significant for solid diffusion due to the adsorbed phase concentration driving force. For pore diffusion, protein mobility is considerable at low and moderate binding strength. Only when pore diffusion if completely dominant, and the binding strength is very high, protein mobility is low enough to restrict diffusion out of the particles. Simulation of column operation reveals substantial protein loss when operating conditions are not adjusted appropriately.
000902465 536__ $$0G:(DE-HGF)POF4-2172$$a2172 - Utilization of renewable carbon and energy sources and engineering of ecosystem functions (POF4-217)$$cPOF4-217$$fPOF IV$$x0
000902465 588__ $$aDataset connected to CrossRef, Journals: juser.fz-juelich.de
000902465 7001_ $$0P:(DE-Juel1)129081$$avon Lieres, Eric$$b1
000902465 7001_ $$00000-0002-4462-2077$$aZaghi, Negar$$b2
000902465 7001_ $$0P:(DE-Juel1)139548$$aLeweke, Samuel$$b3
000902465 7001_ $$0P:(DE-HGF)0$$aCarta, Giorgio$$b4
000902465 7001_ $$0P:(DE-HGF)0$$aHahn, Rainer$$b5$$eCorresponding author
000902465 773__ $$0PERI:(DE-600)1491247-8$$a10.1016/j.chroma.2021.462412$$gVol. 1653, p. 462412 -$$p462412 -$$tJournal of chromatography / A$$v1653$$x0021-9673$$y2021
000902465 8564_ $$uhttps://juser.fz-juelich.de/record/902465/files/1-s2.0-S0021967321005367-main.pdf$$yOpenAccess
000902465 909CO $$ooai:juser.fz-juelich.de:902465$$pdnbdelivery$$pdriver$$pVDB$$popen_access$$popenaire
000902465 9101_ $$0I:(DE-588b)5008462-8$$6P:(DE-Juel1)129081$$aForschungszentrum Jülich$$b1$$kFZJ
000902465 9101_ $$0I:(DE-588b)5008462-8$$6P:(DE-Juel1)139548$$aForschungszentrum Jülich$$b3$$kFZJ
000902465 9131_ $$0G:(DE-HGF)POF4-217$$1G:(DE-HGF)POF4-210$$2G:(DE-HGF)POF4-200$$3G:(DE-HGF)POF4$$4G:(DE-HGF)POF$$9G:(DE-HGF)POF4-2172$$aDE-HGF$$bForschungsbereich Erde und Umwelt$$lErde im Wandel – Unsere Zukunft nachhaltig gestalten$$vFür eine nachhaltige Bio-Ökonomie – von Ressourcen zu Produkten$$x0
000902465 9141_ $$y2021
000902465 915__ $$0StatID:(DE-HGF)0200$$2StatID$$aDBCoverage$$bSCOPUS$$d2021-02-04
000902465 915__ $$0StatID:(DE-HGF)0300$$2StatID$$aDBCoverage$$bMedline$$d2021-02-04
000902465 915__ $$0StatID:(DE-HGF)1050$$2StatID$$aDBCoverage$$bBIOSIS Previews$$d2021-02-04
000902465 915__ $$0StatID:(DE-HGF)1190$$2StatID$$aDBCoverage$$bBiological Abstracts$$d2021-02-04
000902465 915__ $$0StatID:(DE-HGF)0600$$2StatID$$aDBCoverage$$bEbsco Academic Search$$d2021-02-04
000902465 915__ $$0StatID:(DE-HGF)1150$$2StatID$$aDBCoverage$$bCurrent Contents - Physical, Chemical and Earth Sciences$$d2021-02-04
000902465 915__ $$0StatID:(DE-HGF)1030$$2StatID$$aDBCoverage$$bCurrent Contents - Life Sciences$$d2021-02-04
000902465 915__ $$0StatID:(DE-HGF)0113$$2StatID$$aWoS$$bScience Citation Index Expanded$$d2021-02-04
000902465 915__ $$0StatID:(DE-HGF)0150$$2StatID$$aDBCoverage$$bWeb of Science Core Collection$$d2021-02-04
000902465 915__ $$0StatID:(DE-HGF)9900$$2StatID$$aIF < 5$$d2021-02-04
000902465 915__ $$0StatID:(DE-HGF)0510$$2StatID$$aOpenAccess
000902465 915__ $$0StatID:(DE-HGF)0030$$2StatID$$aPeer Review$$bASC$$d2021-02-04
000902465 915__ $$0StatID:(DE-HGF)0100$$2StatID$$aJCR$$bJ CHROMATOGR A : 2019$$d2021-02-04
000902465 915__ $$0StatID:(DE-HGF)0160$$2StatID$$aDBCoverage$$bEssential Science Indicators$$d2021-02-04
000902465 915__ $$0LIC:(DE-HGF)CCBY4$$2HGFVOC$$aCreative Commons Attribution CC BY 4.0
000902465 915__ $$0StatID:(DE-HGF)0420$$2StatID$$aNationallizenz$$d2021-02-04$$wger
000902465 915__ $$0StatID:(DE-HGF)0199$$2StatID$$aDBCoverage$$bClarivate Analytics Master Journal List$$d2021-02-04
000902465 9201_ $$0I:(DE-Juel1)IBG-1-20101118$$kIBG-1$$lBiotechnologie$$x0
000902465 980__ $$ajournal
000902465 980__ $$aVDB
000902465 980__ $$aUNRESTRICTED
000902465 980__ $$aI:(DE-Juel1)IBG-1-20101118
000902465 9801_ $$aFullTexts