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@ARTICLE{Beck:902465,
      author       = {Beck, Jürgen and von Lieres, Eric and Zaghi, Negar and
                      Leweke, Samuel and Carta, Giorgio and Hahn, Rainer},
      title        = {{P}atterns of protein adsorption in ion-exchange particles
                      and columns: {E}volution of protein concentration profiles
                      during load, hold, and wash steps predicted for pore and
                      solid diffusion mechanisms},
      journal      = {Journal of chromatography / A},
      volume       = {1653},
      issn         = {0021-9673},
      address      = {New York, NY [u.a.]},
      publisher    = {Science Direct},
      reportid     = {FZJ-2021-04284},
      pages        = {462412 -},
      year         = {2021},
      abstract     = {Elucidation of protein transport mechanism in ion exchanges
                      is essential to model separation performance. In this work
                      we simulate intraparticle adsorption profiles during batch
                      adsorption assuming typical process conditions for pore,
                      solid and parallel diffusion. Artificial confocal laser
                      scanning microscopy images are created to identify apparent
                      differences between the different transport mechanisms.
                      Typical sharp fronts for pore diffusion are characteristic
                      for Langmuir equilibrium constants of KL ≥1. Only at KL =
                      0.1 and lower, the profiles are smooth and practically
                      indistinguishable from a solid diffusion mechanism. During
                      hold and wash steps, at which the interstitial buffer is
                      removed or exchanged, continuation of diffusion of protein
                      molecules is significant for solid diffusion due to the
                      adsorbed phase concentration driving force. For pore
                      diffusion, protein mobility is considerable at low and
                      moderate binding strength. Only when pore diffusion if
                      completely dominant, and the binding strength is very high,
                      protein mobility is low enough to restrict diffusion out of
                      the particles. Simulation of column operation reveals
                      substantial protein loss when operating conditions are not
                      adjusted appropriately.},
      cin          = {IBG-1},
      ddc          = {540},
      cid          = {I:(DE-Juel1)IBG-1-20101118},
      pnm          = {2172 - Utilization of renewable carbon and energy sources
                      and engineering of ecosystem functions (POF4-217)},
      pid          = {G:(DE-HGF)POF4-2172},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:34320430},
      UT           = {WOS:000677692300009},
      doi          = {10.1016/j.chroma.2021.462412},
      url          = {https://juser.fz-juelich.de/record/902465},
}