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000902470 1001_ $$0P:(DE-HGF)0$$aHüdig, Meike$$b0
000902470 245__ $$aRespiratory and C4-photosynthetic NAD-malic enzyme coexist in bundle sheath cell mitochondria and evolved via association of differentially adapted subunits
000902470 260__ $$aRockville, Md.$$bSoc.$$c2022
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000902470 520__ $$aIn plant mitochondria, nicotinamide adenine dinucleotide phosphate (NAD)-malic enzyme (NAD-ME) has a housekeeping function in malate respiration. In different plant lineages, NAD-ME was independently co-opted in C4 photosynthesis. In the C4 Cleome species Gynandropsis gynandra and Cleome angustifolia, all NAD-ME genes (NAD-MEα, NAD-MEβ1, and NAD-MEβ2) were affected by C4 evolution and are expressed at higher levels than their orthologs in the C3 species Tarenaya hassleriana. In Tarenaya hassleriana, the NAD-ME housekeeping function is performed by two heteromers, NAD-MEα/β1 and NAD-MEα/β2, with similar biochemical properties. In both C4 species, this role is restricted to NAD-MEα/β2. In the C4 species, NAD-MEα/β1 is exclusively present in the leaves, where it accounts for most of the enzymatic activity. GgNAD-MEα/β1 exhibits high catalytic efficiency and is differentially activated by the C4 intermediate aspartate, confirming its role as the C4-decarboxylase. During C4 evolution, NAD-MEβ1 lost its catalytic activity; its contribution to the enzymatic activity results from a stabilizing effect on the associated α-subunit and the adquisition of regulatory properties. We conclude that in bundle sheath cell mitochondria of C4 species, the functions of NAD-ME as C4 photosynthetic decarboxylase and as a housekeeping enzyme coexist and are performed by isoforms that combine the same α subunit with differentially adapted β subunits.
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000902470 7001_ $$0P:(DE-HGF)0$$aTronconi, Marcos A$$b1$$eCorresponding author
000902470 7001_ $$0P:(DE-HGF)0$$aZubimendi, Juan P$$b2
000902470 7001_ $$0P:(DE-HGF)0$$aSage, Tammy L$$b3
000902470 7001_ $$0P:(DE-HGF)0$$aPoschmann, Gereon$$b4
000902470 7001_ $$0P:(DE-HGF)0$$aBickel, David$$b5
000902470 7001_ $$0P:(DE-Juel1)172663$$aGohlke, Holger$$b6$$ufzj
000902470 7001_ $$0P:(DE-HGF)0$$aMaurino, Veronica G$$b7
000902470 773__ $$0PERI:(DE-600)2004373-9$$a10.1093/plcell/koab265$$gp. koab265$$n1$$p597–615$$tThe plant cell$$v34$$x1040-4651$$y2022
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000902470 9141_ $$y2022
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