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@ARTICLE{Wohlers:902929,
      author       = {Wohlers, Karen and Wirtz, Astrid and Reiter, Alexander and
                      Oldiges, Marco and Baumgart, Meike and Bott, Michael},
      title        = {{M}etabolic engineering of {P}seudomonas putida for
                      production of the natural sweetener 5‐ketofructose from
                      fructose or sucrose by periplasmic oxidation with a
                      heterologous fructose dehydrogenase},
      journal      = {Microbial biotechnology},
      volume       = {14},
      number       = {6},
      issn         = {1751-7907},
      address      = {Oxford},
      publisher    = {Wiley-Blackwell},
      reportid     = {FZJ-2021-04682},
      pages        = {2592 - 2604},
      year         = {2021},
      abstract     = {5-Ketofructose (5-KF) is a promising low-calorie natural
                      sweetener with the potential to reduce health problems
                      caused by excessive sugar consumption. It is formed by
                      periplasmic oxidation of fructose by fructose dehydrogenase
                      (Fdh) of Gluconobacter japonicus, a membrane-bound
                      three-subunit enzyme containing FAD and three haemes c as
                      prosthetic groups. This study aimed at establishing
                      Pseudomonas putida KT2440 as a new cell factory for 5-KF
                      production, as this host offers a number of advantages
                      compared with the established host Gluconobacter oxydans.
                      Genomic expression of the fdhSCL genes from G. japonicus
                      enabled synthesis of functional Fdh in P. putida and
                      successful oxidation of fructose to 5-KF. In a batch
                      fermentation, 129 g l−1 5-KF were formed from
                      150 g l−1 fructose within 23 h, corresponding to a
                      space-time yield of 5.6 g l−1 h−1. Besides
                      fructose, also sucrose could be used as substrate for 5-KF
                      production by plasmid-based expression of the invertase gene
                      inv1417 from G. japonicus. In a bioreactor cultivation
                      with pulsed sucrose feeding, 144 g 5-KF were produced from
                      358 g sucrose within 48 h. These results demonstrate
                      that P. putida is an attractive host for 5-KF production.},
      cin          = {IBG-1},
      ddc          = {610},
      cid          = {I:(DE-Juel1)IBG-1-20101118},
      pnm          = {2172 - Utilization of renewable carbon and energy sources
                      and engineering of ecosystem functions (POF4-217)},
      pid          = {G:(DE-HGF)POF4-2172},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:34437751},
      UT           = {WOS:000688662100001},
      doi          = {10.1111/1751-7915.13913},
      url          = {https://juser.fz-juelich.de/record/902929},
}