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@ARTICLE{Krmer:903019,
author = {Krämer, Felicia and Gröner, Benedikt and Hoffmann, Chris
and Craig, Austin and Brugger, Melanie and Drzezga,
Alexander and Timmer, Marco and Neumaier, Felix and
Zlatopolskiy, Boris D. and Endepols, Heike and Neumaier,
Bernd},
title = {{E}valuation of 3-l- and 3-d-[18{F}]{F}luorophenylalanines
as {PET} {T}racers for {T}umor {I}maging},
journal = {Cancers},
volume = {13},
number = {23},
issn = {2072-6694},
address = {Basel},
publisher = {MDPI},
reportid = {FZJ-2021-04748},
pages = {6030 -},
year = {2021},
abstract = {Purpose: The preclinical evaluation of 3-l- and
3-d-[18F]FPhe in comparison to [18F]FET, an established
tracer for tumor imaging. Methods: In vitro studies were
conducted with MCF-7, PC-3, and U87 MG human tumor cell
lines. In vivo µPET studies were conducted in healthy rats
with/without the inhibition of peripheral aromatic l-amino
acid decarboxylase by benserazide pretreatment (n = 3 each),
in mice bearing subcutaneous MCF-7 or PC-3 tumor xenografts
(n = 10), and in rats bearing orthotopic U87 MG tumor
xenografts (n = 14). Tracer accumulation was quantified by
SUVmax, SUVmean and tumor-to-brain ratios (TBrR). Results:
The uptake of 3-l-[18F]FPhe in MCF-7 and PC-3 cells was
significantly higher relative to [18F]FET. The uptake of all
three tracers was significantly reduced by the suppression
of amino acid transport systems L or ASC. 3-l-[18F]FPhe but
not 3-d-[18F]FPhe exhibited protein incorporation. In
benserazide-treated healthy rats, brain uptake after
42–120 min was significantly higher for 3-d-[18F]FPhe vs.
3-l-[18F]FPhe. [18F]FET showed significantly higher uptake
into subcutaneous MCF-7 tumors (52–60 min p.i.), while
early uptake into orthotopic U87 MG tumors was significantly
higher for 3-l-[18F]FPhe (SUVmax: 3-l-[18F]FPhe, 107.6 ±
11.3; 3-d-[18F]FPhe, 86.0 ± 4.3; [18F]FET, 90.2 ± 7.7).
Increased tumoral expression of LAT1 and ASCT2 was confirmed
immunohistologically. Conclusion: Both novel tracers enable
accurate tumor delineation with an imaging quality
comparable to [18F]FET.},
cin = {INM-5 / INM-2},
ddc = {610},
cid = {I:(DE-Juel1)INM-5-20090406 / I:(DE-Juel1)INM-2-20090406},
pnm = {5253 - Neuroimaging (POF4-525)},
pid = {G:(DE-HGF)POF4-5253},
typ = {PUB:(DE-HGF)16},
pubmed = {34885141},
UT = {WOS:000734552300001},
doi = {10.3390/cancers13236030},
url = {https://juser.fz-juelich.de/record/903019},
}