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000903040 1001_ $$0P:(DE-Juel1)179367$$aLee, Namkyu$$b0$$eFirst author
000903040 245__ $$aTemperature profile characterization with fluorescence lifetime imaging microscopy in a thermophoretic chip
000903040 260__ $$aHeidelberg$$bSpringer$$c2021
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000903040 520__ $$aThis study introduces a thermophoretic lab-on-a-chip device to measure the Soret coefficient. We use resistive heating of a microwire on the chip to induce a temperature gradient, which is measured by fluorescence lifetime imaging microscopy (FLIM). To verify the functionality of the device, we used dyed polystyrene particles with a diameter of 25 nm. A confocal microscope is utilized to monitor the concentration profile of colloidal particles in the temperature field. Based on the measured temperature and concentration differences, we calculate the corresponding Soret coefficient. The same particles have been recently investigated with thermal diffusion forced Rayleigh scattering (TDFRS) and we find that the obtained Soret coefficients agree with literature results. This chip offers a simple way to study the thermophoretic behavior of biological systems in multicomponent buffer solutions quantitatively, which are difficult to study with optical methods solely relying on the refractive index contrast. 
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000903040 7001_ $$0P:(DE-Juel1)144600$$aAfanasenkau, Dzmitry$$b1
000903040 7001_ $$0P:(DE-Juel1)140264$$aRinklin, Philipp$$b2
000903040 7001_ $$0P:(DE-Juel1)128745$$aWolfrum, Bernhard$$b3
000903040 7001_ $$0P:(DE-Juel1)131034$$aWiegand, Simone$$b4$$eCorresponding author
000903040 773__ $$0PERI:(DE-600)2004003-9$$a10.1140/epje/s10189-021-00133-7$$gVol. 44, no. 10, p. 130$$n10$$p130$$tThe European physical journal / E$$v44$$x1292-8941$$y2021
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