TY  - JOUR
AU  - Lee, Namkyu
AU  - Afanasenkau, Dzmitry
AU  - Rinklin, Philipp
AU  - Wolfrum, Bernhard
AU  - Wiegand, Simone
TI  - Temperature profile characterization with fluorescence lifetime imaging microscopy in a thermophoretic chip
JO  - The European physical journal / E
VL  - 44
IS  - 10
SN  - 1292-8941
CY  - Heidelberg
PB  - Springer
M1  - FZJ-2021-04769
SP  - 130
PY  - 2021
AB  - This study introduces a thermophoretic lab-on-a-chip device to measure the Soret coefficient. We use resistive heating of a microwire on the chip to induce a temperature gradient, which is measured by fluorescence lifetime imaging microscopy (FLIM). To verify the functionality of the device, we used dyed polystyrene particles with a diameter of 25 nm. A confocal microscope is utilized to monitor the concentration profile of colloidal particles in the temperature field. Based on the measured temperature and concentration differences, we calculate the corresponding Soret coefficient. The same particles have been recently investigated with thermal diffusion forced Rayleigh scattering (TDFRS) and we find that the obtained Soret coefficients agree with literature results. This chip offers a simple way to study the thermophoretic behavior of biological systems in multicomponent buffer solutions quantitatively, which are difficult to study with optical methods solely relying on the refractive index contrast. 
LB  - PUB:(DE-HGF)16
C6  - 34668081
UR  - <Go to ISI:>//WOS:000708879000001
DO  - DOI:10.1140/epje/s10189-021-00133-7
UR  - https://juser.fz-juelich.de/record/903040
ER  -