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@ARTICLE{Lee:903040,
      author       = {Lee, Namkyu and Afanasenkau, Dzmitry and Rinklin, Philipp
                      and Wolfrum, Bernhard and Wiegand, Simone},
      title        = {{T}emperature profile characterization with fluorescence
                      lifetime imaging microscopy in a thermophoretic chip},
      journal      = {The European physical journal / E},
      volume       = {44},
      number       = {10},
      issn         = {1292-8941},
      address      = {Heidelberg},
      publisher    = {Springer},
      reportid     = {FZJ-2021-04769},
      pages        = {130},
      year         = {2021},
      abstract     = {This study introduces a thermophoretic lab-on-a-chip device
                      to measure the Soret coefficient. We use resistive heating
                      of a microwire on the chip to induce a temperature gradient,
                      which is measured by fluorescence lifetime imaging
                      microscopy (FLIM). To verify the functionality of the
                      device, we used dyed polystyrene particles with a diameter
                      of 25 nm. A confocal microscope is utilized to monitor the
                      concentration profile of colloidal particles in the
                      temperature field. Based on the measured temperature and
                      concentration differences, we calculate the corresponding
                      Soret coefficient. The same particles have been recently
                      investigated with thermal diffusion forced Rayleigh
                      scattering (TDFRS) and we find that the obtained Soret
                      coefficients agree with literature results. This chip offers
                      a simple way to study the thermophoretic behavior of
                      biological systems in multicomponent buffer solutions
                      quantitatively, which are difficult to study with optical
                      methods solely relying on the refractive index contrast.},
      cin          = {IBI-4},
      ddc          = {530},
      cid          = {I:(DE-Juel1)IBI-4-20200312},
      pnm          = {5241 - Molecular Information Processing in Cellular Systems
                      (POF4-524)},
      pid          = {G:(DE-HGF)POF4-5241},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {34668081},
      UT           = {WOS:000708879000001},
      doi          = {10.1140/epje/s10189-021-00133-7},
      url          = {https://juser.fz-juelich.de/record/903040},
}