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@ARTICLE{Zhang:903142,
author = {Zhang, Zhi-Hui and Hartner, Elena and Utinger, Battist and
Gfeller, Benjamin and Paul, Andreas and Sklorz, Martin and
Czech, Hendryk and Yang, Bin Xia and Su, Xin Yi and Jakobi,
Gert and Orasche, Jürgen and Schnelle-Kreis, Jürgen and
Jeong, Seongho and Gröger, Thomas and Pardo, Michal and
Hohaus, Thorsten and Adam, Thomas and Kiendler-Scharr,
Astrid and Rudich, Yinon and Zimmermann, Ralf and Kalberer,
Markus},
title = {{A}re reactive oxygen species ({ROS}) a suitable metric to
predict toxicity of carbonaceous aerosol particles?},
journal = {Atmospheric chemistry and physics / Discussions},
issn = {1680-7367},
address = {Katlenburg-Lindau},
publisher = {EGU},
reportid = {FZJ-2021-04866},
year = {2021},
abstract = {Abstract. It is being suggested that particle-bound or
particle-induced reactive oxygen species (ROS), which
significantly contribute to the oxidative potential (OP) of
aerosol particles, are a promising metric linking aerosol
compositions to toxicity and adverse health effects.
However, accurate ROS quantification remains challenging due
to the reactive and short-lived nature of many ROS
components and the lack of appropriate analytical methods
for a reliable quantification. Consequently, it remains
difficult to gauge their impact on human health, especially
to identify how aerosol particle sources and atmospheric
processes drive particle-bound ROS formation in a real-world
urban environment. In this study, using a novel online
particle-bound ROS instrument (OPROSI), we comprehensively
characterized and compared the formation of ROS in secondary
organic aerosols (SOA) generated from organic compounds that
represent anthropogenic (naphthalene, SOANAP) and biogenic
(β-pinene, SOAβPIN) precursors. The SOA mass was condensed
onto soot particles (SP) under varied atmospherically
relevant conditions (photochemical aging and humidity). We
systematically analysed the ability of the aqueous extracts
of the two aerosol types (SOANAP-SP and SOAβPIN-SP) to
induce ROS production and OP. We further investigated
cytotoxicity and cellular ROS production after exposing
human lung epithelial cell cultures (A549) to extracts of
the two aerosols. A significant finding of this study is
that more than 90 $\%$ of all ROS components in both SOA
types have a short lifetime, highlighting the need to
develop online instruments for a meaningful quantification
of ROS. Our results also show that photochemical aging
promotes particle-bound ROS production and enhances the OP
of the aerosols. Compared to SOAβPIN-SP, SOANAP-SP elicited
a higher acellular and cellular ROS production, a higher OP
and a lower cell viability. These consistent results between
chemical-based and biological-based analyses indicate that
particle-bound ROS quantification could be a feasible metric
to predict aerosol particle toxicity and adverse human
effects. Moreover, the cellular ROS production caused by SOA
exposure not only depends on aerosol type, but is also
affected by exposure dose, highlighting a need to mimic the
process of particle deposition onto lung cells and their
interactions as realistically as possible to avoid unknown
biases.},
cin = {IEK-8},
ddc = {550},
cid = {I:(DE-Juel1)IEK-8-20101013},
pnm = {2111 - Air Quality (POF4-211)},
pid = {G:(DE-HGF)POF4-2111},
typ = {PUB:(DE-HGF)25},
doi = {10.5194/acp-2021-666},
url = {https://juser.fz-juelich.de/record/903142},
}