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@ARTICLE{Ramm:903752,
author = {Ramm, Ingrid and Sanchez-Fernandez, Adrian and Choi,
Jaeyeong and Lang, Christian and Schagerlöf, Herje and
Wahlgren, Marie and Nilsson, Lars},
title = {{T}he {I}mpact of {G}lycerol on an {A}ffibody
{C}onformation and {I}ts {C}orrelation to {C}hemical
{D}egradation},
journal = {Pharmaceutics},
volume = {13},
number = {11},
issn = {1999-4923},
address = {Basel},
publisher = {MDPI},
reportid = {FZJ-2021-05392},
pages = {1853 -},
year = {2021},
abstract = {The addition of glycerol to protein solutions is often used
to hinder the aggregation and denaturation of proteins.
However, it is not a generalised practice against chemical
degradation reactions. The chemical degradation of proteins,
such as deamidation and isomerisation, is an important
deteriorative mechanism that leads to a loss of
functionality of pharmaceutical proteins. Here, the
influence of glycerol on the chemical degradation of a
protein and its correlation to glycerol-induced
conformational changes is presented. The time-dependent
chemical degradation of a pharmaceutical protein, GA-Z, in
the absence and presence of glycerol was investigated in a
stability study. The effect of glycerol on protein
conformation and oligomerisation was characterised using
asymmetric field-flow fractionation and small-angle neutron
scattering in a wide glycerol concentration range of
$0–90\%$ v/v. The results from the stability study were
connected to the observed glycerol-induced conformational
changes in the protein. A correlation between protein
conformation and the protective effect of glycerol against
the degradation reactions deamidation, isomerisation, and
hydrolysis was found. The study reveals that glycerol
induces conformational changes of the protein, which favour
a more compact and chemically stable state. It is also shown
that the conformation can be changed by other system
properties, e.g., protein concentration, leading to
increased chemical stability.},
cin = {JCNS-FRM-II / JCNS-1 / JCNS-4 / MLZ},
ddc = {610},
cid = {I:(DE-Juel1)JCNS-FRM-II-20110218 /
I:(DE-Juel1)JCNS-1-20110106 / I:(DE-Juel1)JCNS-4-20201012 /
I:(DE-588b)4597118-3},
pnm = {6G4 - Jülich Centre for Neutron Research (JCNS) (FZJ)
(POF4-6G4) / 632 - Materials – Quantum, Complex and
Functional Materials (POF4-632)},
pid = {G:(DE-HGF)POF4-6G4 / G:(DE-HGF)POF4-632},
experiment = {EXP:(DE-MLZ)KWS2-20140101},
typ = {PUB:(DE-HGF)16},
pubmed = {34834267},
UT = {WOS:000792960900014},
doi = {10.3390/pharmaceutics13111853},
url = {https://juser.fz-juelich.de/record/903752},
}