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@ARTICLE{Bernardes:904307,
author = {Bernardes, Juliana S. and Eberle, Raphael J. and Vieira,
Fabio R. J. and Coronado, Mônika A.},
title = {{A} comparative pan-genomic analysis of 53 {C}.
pseudotuberculosis strains based on functional domains},
journal = {Journal of biomolecular structure $\&$ dynamics},
volume = {39},
number = {18},
issn = {0739-1102},
address = {Abingdon [u.a.]},
publisher = {Taylor $\&$ Francis},
reportid = {FZJ-2021-05877},
pages = {6974 - 6986},
year = {2021},
abstract = {Corynebacterium pseudotuberculosis is a pathogenic
bacterium with great veterinary and economic importance. It
is classified into two biovars: ovis, nitrate-negative, that
causes lymphadenitis in small ruminants and equi,
nitrate-positive, causing ulcerative lymphangitis in
equines. With the explosive growth of available genomes of
several strains, pan-genome analysis has opened new
opportunities for understanding the dynamics and evolution
of C. pseudotuberculosis. However, few pan-genomic studies
have compared biovars equi and ovis. Such studies have
considered a reduced number of strains and compared entire
genomes. Here we conducted an original pan-genome analysis
based on protein sequences and their functional domains. We
considered 53 C. pseudotuberculosis strains from both
biovars isolated from different hosts and countries. We have
analysed conserved domains, common domains more frequently
found in each biovar and biovar-specific (unique) domains.
Our results demonstrated that biovar equi is more variable;
there is a significant difference in the number of proteins
per strains, probably indicating the occurrence of more gene
loss/gain events. Moreover, strains of biovar equi presented
a higher number of biovar-specific domains, 77 against only
eight in biovar ovis, most of them are associated with
virulence mechanisms. With this domain analysis, we have
identified functional differences among strains of biovars
ovis and equi that could be related to niche-adaptation and
probably help to better understanding mechanisms of
virulence and pathogenesis. The distribution patterns of
functional domains identified in this work might have
impacts on bacterial physiology and lifestyle, encouraging
the development of new diagnoses, vaccines, and treatments
for C. pseudotuberculosis diseases.Communicated by Ramaswamy
H. Sarma.},
cin = {IBI-7},
ddc = {570},
cid = {I:(DE-Juel1)IBI-7-20200312},
pnm = {5244 - Information Processing in Neuronal Networks
(POF4-524)},
pid = {G:(DE-HGF)POF4-5244},
typ = {PUB:(DE-HGF)16},
pubmed = {32779519},
UT = {WOS:000557983700001},
doi = {10.1080/07391102.2020.1805017},
url = {https://juser.fz-juelich.de/record/904307},
}