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@ARTICLE{Dubey:904309,
      author       = {Dubey, Abhinav and Stoyanov, Nikolay and Viennet, Thibault
                      and Chhabra, Sandeep and Elter, Shantha and Borggräfe, Jan
                      and Viegas, Aldino and Nowak, Radosław P. and Burdzhiev,
                      Nikola and Petrov, Ognyan and Fischer, Eric S. and Etzkorn,
                      Manuel and Gelev, Vladimir and Arthanari, Haribabu},
      title        = {{L}ocal {D}euteration {E}nables {NMR} {O}bservation of
                      {M}ethyl {G}roups in {P}roteins from {E}ukaryotic and
                      {C}ell‐{F}ree {E}xpression {S}ystems},
      journal      = {Angewandte Chemie / International edition},
      volume       = {60},
      number       = {25},
      issn         = {0570-0833},
      address      = {Weinheim},
      publisher    = {Wiley-VCH},
      reportid     = {FZJ-2021-05879},
      pages        = {13783 - 13787},
      year         = {2021},
      abstract     = {Therapeutically relevant proteins such as GPCRs, antibodies
                      and kinases face clear limitations in NMR studies due to the
                      challenges in site-specific isotope labeling and deuteration
                      in eukaryotic expression systems. Here we describe an
                      efficient and simple method to observe the methyl groups of
                      leucine residues in proteins expressed in bacterial,
                      eukaryotic or cell-free expression systems without
                      modification of the expression protocol. The method relies
                      on simple stereo-selective 13C-labeling and deuteration of
                      leucine that alleviates the need for additional deuteration
                      of the protein. The spectroscopic benefits of “local”
                      deuteration are examined in detail through Forbidden
                      Coherence Transfer (FCT) experiments and simulations. The
                      utility of this labeling method is demonstrated in the
                      cell-free synthesis of bacteriorhodopsin and in the
                      insect-cell expression of the RRM2 domain of human RBM39.},
      cin          = {IBI-7},
      ddc          = {540},
      cid          = {I:(DE-Juel1)IBI-7-20200312},
      pnm          = {5244 - Information Processing in Neuronal Networks
                      (POF4-524)},
      pid          = {G:(DE-HGF)POF4-5244},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:33768661},
      UT           = {WOS:000647987600001},
      doi          = {10.1002/anie.202016070},
      url          = {https://juser.fz-juelich.de/record/904309},
}