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@ARTICLE{Zielinski:904314,
      author       = {Zielinski, Mara and Röder, Christine and Schröder, Gunnar
                      F.},
      title        = {{C}hallenges in sample preparation and structure
                      determination of amyloids by cryo-{EM}},
      journal      = {The journal of biological chemistry},
      volume       = {297},
      number       = {2},
      issn         = {0021-9258},
      address      = {Bethesda, Md.},
      publisher    = {Soc.},
      reportid     = {FZJ-2021-05884},
      pages        = {100938 -},
      year         = {2021},
      abstract     = {Amyloids share a common architecture but play disparate
                      biological roles in processes ranging from bacterial defense
                      mechanisms to protein misfolding diseases. Their structures
                      are highly polymorphic, which makes them difficult to study
                      by X-ray diffraction or NMR spectroscopy. Our understanding
                      of amyloid structures is due in large part to recent
                      advances in the field of cryo-EM, which allows for
                      determining the polymorphs separately. In this review, we
                      highlight the main stepping stones leading to the
                      substantial number of high-resolution amyloid fibril
                      structures known today as well as recent developments
                      regarding automation and software in cryo-EM. We discuss
                      that sample preparation should move closer to physiological
                      conditions to understand how amyloid aggregation and disease
                      are linked. We further highlight new approaches to address
                      heterogeneity and polymorphism of amyloid fibrils in EM
                      image processing and give an outlook to the upcoming
                      challenges in researching the structural biology of
                      amyloids.},
      cin          = {IBI-7},
      ddc          = {540},
      cid          = {I:(DE-Juel1)IBI-7-20200312},
      pnm          = {5244 - Information Processing in Neuronal Networks
                      (POF4-524)},
      pid          = {G:(DE-HGF)POF4-5244},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:34224730},
      UT           = {WOS:000690871000008},
      doi          = {10.1016/j.jbc.2021.100938},
      url          = {https://juser.fz-juelich.de/record/904314},
}