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@ARTICLE{Yaroshevich:904316,
author = {Yaroshevich, Igor A. and Maksimov, Eugene G. and Sluchanko,
Nikolai N. and Zlenko, Dmitry V. and Stepanov, Alexey V. and
Slutskaya, Ekaterina A. and Slonimskiy, Yury B. and
Botnarevskii, Viacheslav S. and Remeeva, Alina and Gushchin,
Ivan and Kovalev, Kirill and Gordeliy, Valentin I. and
Shelaev, Ivan V. and Gostev, Fedor E. and Khakhulin, Dmitry
and Poddubnyy, Vladimir V. and Gostev, Timofey S. and
Cherepanov, Dmitry A. and Polívka, Tomáš and Kloz,
Miroslav and Friedrich, Thomas and Paschenko, Vladimir Z.
and Nadtochenko, Victor A. and Rubin, Andrew B. and
Kirpichnikov, Mikhail P.},
title = {{R}ole of hydrogen bond alternation and charge transfer
states in photoactivation of the {O}range {C}arotenoid
{P}rotein},
journal = {Communications biology},
volume = {4},
number = {1},
issn = {2399-3642},
address = {London},
publisher = {Springer Nature},
reportid = {FZJ-2021-05886},
pages = {539},
year = {2021},
abstract = {Here, we propose a possible photoactivation mechanism of a
35-kDa blue light-triggered photoreceptor, the Orange
Carotenoid Protein (OCP), suggesting that the reaction
involves the transient formation of a protonated
ketocarotenoid (oxocarbenium cation) state. Taking advantage
of engineering an OCP variant carrying the Y201W mutation,
which shows superior spectroscopic and structural
properties, it is shown that the presence of Trp201 augments
the impact of one critical H-bond between the ketocarotenoid
and the protein. This confers an unprecedented homogeneity
of the dark-adapted OCP state and substantially increases
the yield of the excited photoproduct S*, which is important
for the productive photocycle to proceed. A 1.37 Å
crystal structure of OCP Y201W combined with femtosecond
time-resolved absorption spectroscopy, kinetic analysis, and
deconvolution of the spectral intermediates, as well as
extensive quantum chemical calculations incorporating the
effect of the local electric field, highlighted the role of
charge-transfer states during OCP photoconversion.},
cin = {IBI-7},
ddc = {570},
cid = {I:(DE-Juel1)IBI-7-20200312},
pnm = {5241 - Molecular Information Processing in Cellular Systems
(POF4-524)},
pid = {G:(DE-HGF)POF4-5241},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:33972665},
UT = {WOS:000656215600008},
doi = {10.1038/s42003-021-02022-3},
url = {https://juser.fz-juelich.de/record/904316},
}