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@ARTICLE{Xu:904317,
      author       = {Xu, Amy Y. and McGillivray, Duncan J. and Dingley, Andrew
                      J.},
      title        = {{A}ctive antibacterial coating of cotton fabrics with
                      antimicrobial proteins},
      journal      = {Cellulose},
      volume       = {28},
      number       = {12},
      issn         = {0969-0239},
      address      = {Dordrecht [u.a.]},
      publisher    = {Springer Science + Business Media B.V},
      reportid     = {FZJ-2021-05887},
      pages        = {8077 - 8094},
      year         = {2021},
      abstract     = {The prevention of bacteria colonization by immobilizing
                      proteins with antimicrobial activity onto cotton fabrics was
                      investigated. Such coatings have potential applications in
                      medical dressing materials used in wound care and healing.
                      Two antimicrobial proteins lysozyme and hydramacin-1 (HM-1)
                      were surface immobilized through two linkers (3-aminopropyl)
                      triethoxysilane (APTES) and citric acid in the presence of
                      the water soluble carbodiimide coupling reagent
                      1-cyclohexyl-3-(2-morpholinoethyl)carbodiimide
                      metho-p-toluenesulfonate. Surface composition analysis by
                      attenuated total reflection-Fourier transform infrared and
                      X-ray photoelectron spectroscopies confirmed formation of
                      the protein-cellulose conjugates. Antimicrobial activities
                      of the different functionalized surfaces were found to vary
                      between APTES and citric acid directed coatings. Citric acid
                      immobilized lysozyme treated samples demonstrated superior
                      activity against Gram-positive Bacillus subtilis, whereas
                      APTES immobilized HM-1 treated samples demonstrated an
                      advantage in inhibiting the growth of Gram-negative
                      Escherichia coli. The antibacterial activity and stability
                      of citric acid immobilized protein fabrics following
                      sonication, boiling and chemical treatment were noticeably
                      higher than that of the corresponding APTES immobilized
                      protein fabrics. The dual coating of fibers with both
                      antimicrobial proteins afforded efficient antimicrobial
                      activities against both bacterial species. The results
                      suggest that coating cotton fibers with antimicrobial
                      proteins and peptides represents a feasible approach for
                      developing active surfaces that prohibit growth and
                      colonization of bacterial strains and can be potentially
                      used in medical cotton-based fabrics.},
      cin          = {IBI-7},
      ddc          = {540},
      cid          = {I:(DE-Juel1)IBI-7-20200312},
      pnm          = {5244 - Information Processing in Neuronal Networks
                      (POF4-524)},
      pid          = {G:(DE-HGF)POF4-5244},
      typ          = {PUB:(DE-HGF)16},
      UT           = {WOS:000664442100002},
      doi          = {10.1007/s10570-021-03997-2},
      url          = {https://juser.fz-juelich.de/record/904317},
}