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@ARTICLE{Rokitskaya:904325,
author = {Rokitskaya, Tatyana I. and Maliar, Nina and Kovalev, Kirill
V. and Volkov, Oleksandr and Gordeliy, Valentin I. and
Antonenko, Yuri N.},
title = {{R}hodopsin {C}hannel {A}ctivity {C}an {B}e {E}valuated by
{M}easuring the {P}hotocurrent {V}oltage {D}ependence in
{P}lanar {B}ilayer {L}ipid {M}embranes},
journal = {Biochemistry (Moscow)},
volume = {86},
number = {4},
issn = {0006-2979},
address = {Dordrecht [u.a.]},
publisher = {Springer Science + Business Media B.V},
reportid = {FZJ-2021-05895},
pages = {409 - 419},
year = {2021},
abstract = {The studies of the functional properties of
retinal-containing proteins often include experiments in
model membrane systems, e.g., measurements of electric
current through planar bilayer lipid membranes (BLMs) with
proteoliposomes adsorbed on one of the membrane surfaces.
However, the possibilities of this method have not been
fully explored yet. We demonstrated that the voltage
dependence of stationary photocurrents for two
light-sensitive proteins, bacteriorhodopsin (bR) and
channelrhodopsin 2 (ChR2), in the presence of protonophore
had very different characteristics. In the case of the bR
(proton pump), the photocurrent through the BLM did not
change direction when the polarity of the applied voltage
was switched. In the case of the photosensitive channel
protein ChR2, the photocurrent increased with the increase
in voltage and the current polarity changed with the change
in the voltage polarity. The protonophore
4,5,6,7-tetrachloro-2-trifluoromethyl benzimidazole (TTFB)
was more efficient in the maximizing stationary
photocurrents. In the presence of carbonyl
cyanide-m-chlorophenylhydrazone (CCCP), the amplitude of the
measured photocurrents for bR significantly decreased, while
in the case of ChR2, the photocurrents virtually
disappeared. The difference between the effects of TTFB and
CCCP was apparently due to the fact that, in contrast to
TTFB, CCCP transfers protons across the liposome membranes
with a higher rate than through the decane-containing BLM
used as a surface for the proteoliposome adsorption.},
cin = {IBI-7},
ddc = {540},
cid = {I:(DE-Juel1)IBI-7-20200312},
pnm = {5241 - Molecular Information Processing in Cellular Systems
(POF4-524)},
pid = {G:(DE-HGF)POF4-5241},
typ = {PUB:(DE-HGF)16},
pubmed = {33941063},
UT = {WOS:000630849000001},
doi = {10.1134/S0006297921040039},
url = {https://juser.fz-juelich.de/record/904325},
}