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@ARTICLE{Wu:904340,
      author       = {Wu, Changtong and Barkova, Daria and Komarova, Natalia and
                      Offenhäusser, Andreas and Andrianova, Mariia and Hu, Ziheng
                      and Kuznetsov, Alexander and Mayer, Dirk},
      title        = {{H}ighly selective and sensitive detection of glutamate by
                      an electrochemical aptasensor},
      journal      = {Fresenius' journal of analytical chemistry},
      volume       = {44},
      issn         = {0016-1152},
      address      = {Berlin},
      publisher    = {Springer},
      reportid     = {FZJ-2021-05910},
      pages        = {1609–1622},
      year         = {2022},
      abstract     = {An electrochemical aptamer-based sensor was developed for
                      glutamate, the major excitatory neurotransmitter in the
                      central nervous system. Determining glutamic acid release
                      and glutamic acid levels is crucial for studying signal
                      transmission and for diagnosing pathological conditions in
                      the brain. Glutamic acid-selective oligonucleotides were
                      isolated from an ssDNA library using the Capture-SELEX
                      protocol in complex medium. The selection permitted the
                      isolation of an aptamer 1d04 with a dissociation constant of
                      12 µM. The aptamer sequence was further used in the
                      development of an electrochemical aptamer sensor. For this
                      purpose, a truncated aptamer sequence named glu1 was
                      labelled with a ferrocene redox tag at the 3′-end and
                      immobilized on a gold electrode surface via Au-thiol bonds.
                      Using 6-mercapto-1-hexanol as the backfill, the sensor
                      performance was characterized by alternating current
                      voltammetry. The glu1 aptasensor showed a limit of detection
                      of 0.0013 pM, a wide detection range between 0.01 pM and 1
                      nM, and good selectivity for glutamate in tenfold diluted
                      human serum. With this enzyme-free aptasensor, the highly
                      selective and sensitive detection of glutamate was
                      demonstrated, which possesses great potential for
                      implementation in microelectrodes and for in vitro as well
                      as in vivo monitoring of neurotransmitter release.},
      cin          = {IBI-3},
      ddc          = {540},
      cid          = {I:(DE-Juel1)IBI-3-20200312},
      pnm          = {5244 - Information Processing in Neuronal Networks
                      (POF4-524)},
      pid          = {G:(DE-HGF)POF4-5244},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {34783880},
      UT           = {WOS:000719180700002},
      doi          = {10.1007/s00216-021-03783-w},
      url          = {https://juser.fz-juelich.de/record/904340},
}