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@ARTICLE{Wolf:904341,
author = {Wolf, Nikolaus and Rai, Pratika and Glass, Manuel and
Milos, Frano and Maybeck, Vanessa and Offenhäusser, Andreas
and Wördenweber, Roger},
title = {{M}echanical and {E}lectronic {C}ell–{C}hip {I}nteraction
of {APTES}-{F}unctionalized {N}euroelectronic {I}nterfaces},
journal = {ACS applied bio materials},
volume = {4},
number = {8},
issn = {2576-6422},
address = {Washington, DC},
publisher = {ACS Publications},
reportid = {FZJ-2021-05911},
pages = {6326 - 6337},
year = {2021},
abstract = {In this work, we analyze the impact of a chip coating with
a self-assembled monolayer (SAM) of
(3-aminopropyl)triethoxysilane (APTES) on the electronic and
mechanical properties of neuroelectronic interfaces. We show
that the large signal transfer, which has been observed for
these interfaces, is most likely a consequence of the strong
mechanical coupling between cells and substrate. On the one
hand, we demonstrate that the impedance of the interface
between Pt electrodes and an electrolyte is slightly reduced
by the APTES SAM. However, this reduction of approximately
$13\%$ is definitely not sufficient to explain the large
signal transfer of APTES coated electrodes demonstrated
previously. On the other hand, the APTES coating leads to a
stronger mechanical clamping of the cells, which is visible
in microscopic images of the cell development of
APTES-coated substrates. This stronger mechanical
interaction is most likely caused by the positively charged
amino functional group of the APTES SAM. It seems to lead to
a smaller cleft between substrate and cells and, thus, to
reduced losses of the cell’s action potential signal at
the electrode. The disadvantage of this tight binding of the
cells to the rigid, planar substrate seems to be the short
lifetime of the cells. In our case the density of living
cells starts to decrease together with the visual
deformation of the cells typically at DIV 9. Solutions for
this problem might be the use of soft substrates and/or the
replacement of the short APTES molecules with larger
molecules or molecular multilayers.},
cin = {IBI-3},
ddc = {570},
cid = {I:(DE-Juel1)IBI-3-20200312},
pnm = {5244 - Information Processing in Neuronal Networks
(POF4-524)},
pid = {G:(DE-HGF)POF4-5244},
typ = {PUB:(DE-HGF)16},
pubmed = {35006867},
UT = {WOS:000687042400043},
doi = {10.1021/acsabm.1c00576},
url = {https://juser.fz-juelich.de/record/904341},
}