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000904342 1001_ $$0P:(DE-HGF)0$$aWelden, Rene$$b0
000904342 245__ $$aLight-Addressable Actuator-Sensor Platform for Monitoring and Manipulation of pH Gradients in Microfluidics: A Case Study with the Enzyme Penicillinase
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000904342 520__ $$aThe feasibility of light-addressed detection and manipulation of pH gradients inside an electrochemical microfluidic cell was studied. Local pH changes, induced by a light-addressable electrode (LAE), were detected using a light-addressable potentiometric sensor (LAPS) with different measurement modes representing an actuator-sensor system. Biosensor functionality was examined depending on locally induced pH gradients with the help of the model enzyme penicillinase, which had been immobilized in the microfluidic channel. The surface morphology of the LAE and enzyme-functionalized LAPS was studied by scanning electron microscopy. Furthermore, the penicillin sensitivity of the LAPS inside the microfluidic channel was determined with regard to the analyte’s pH influence on the enzymatic reaction rate. In a final experiment, the LAE-controlled pH inhibition of the enzyme activity was monitored by the LAPS
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000904342 7001_ $$0P:(DE-HGF)0$$aJablonski, Melanie$$b1
000904342 7001_ $$0P:(DE-HGF)0$$aWege, Christina$$b2
000904342 7001_ $$0P:(DE-HGF)0$$aKeusgen, Michael$$b3
000904342 7001_ $$0P:(DE-HGF)0$$aWagner, Patrick Hermann$$b4
000904342 7001_ $$0P:(DE-HGF)0$$aWagner, Torsten$$b5
000904342 7001_ $$0P:(DE-Juel1)128727$$aSchöning, Michael J.$$b6$$eCorresponding author
000904342 773__ $$0PERI:(DE-600)2662125-3$$a10.3390/bios11060171$$gVol. 11, no. 6, p. 171 -$$n6$$p171 -$$tBiosensors$$v11$$x2079-6374$$y2021
000904342 8564_ $$uhttps://juser.fz-juelich.de/record/904342/files/biosensors-11-00171-v2.pdf$$yOpenAccess
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000904342 9141_ $$y2022
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