TY  - JOUR
AU  - Welden, Rene
AU  - Jablonski, Melanie
AU  - Wege, Christina
AU  - Keusgen, Michael
AU  - Wagner, Patrick Hermann
AU  - Wagner, Torsten
AU  - Schöning, Michael J.
TI  - Light-Addressable Actuator-Sensor Platform for Monitoring and Manipulation of pH Gradients in Microfluidics: A Case Study with the Enzyme Penicillinase
JO  - Biosensors
VL  - 11
IS  - 6
SN  - 2079-6374
CY  - Basel
PB  - MDPI
M1  - FZJ-2021-05912
SP  - 171 -
PY  - 2021
AB  - The feasibility of light-addressed detection and manipulation of pH gradients inside an electrochemical microfluidic cell was studied. Local pH changes, induced by a light-addressable electrode (LAE), were detected using a light-addressable potentiometric sensor (LAPS) with different measurement modes representing an actuator-sensor system. Biosensor functionality was examined depending on locally induced pH gradients with the help of the model enzyme penicillinase, which had been immobilized in the microfluidic channel. The surface morphology of the LAE and enzyme-functionalized LAPS was studied by scanning electron microscopy. Furthermore, the penicillin sensitivity of the LAPS inside the microfluidic channel was determined with regard to the analyte’s pH influence on the enzymatic reaction rate. In a final experiment, the LAE-controlled pH inhibition of the enzyme activity was monitored by the LAPS
LB  - PUB:(DE-HGF)16
C6  - 34072213
UR  - <Go to ISI:>//WOS:000665493000001
DO  - DOI:10.3390/bios11060171
UR  - https://juser.fz-juelich.de/record/904342
ER  -