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@ARTICLE{Welden:904342,
      author       = {Welden, Rene and Jablonski, Melanie and Wege, Christina and
                      Keusgen, Michael and Wagner, Patrick Hermann and Wagner,
                      Torsten and Schöning, Michael J.},
      title        = {{L}ight-{A}ddressable {A}ctuator-{S}ensor {P}latform for
                      {M}onitoring and {M}anipulation of p{H} {G}radients in
                      {M}icrofluidics: {A} {C}ase {S}tudy with the {E}nzyme
                      {P}enicillinase},
      journal      = {Biosensors},
      volume       = {11},
      number       = {6},
      issn         = {2079-6374},
      address      = {Basel},
      publisher    = {MDPI},
      reportid     = {FZJ-2021-05912},
      pages        = {171 -},
      year         = {2021},
      abstract     = {The feasibility of light-addressed detection and
                      manipulation of pH gradients inside an electrochemical
                      microfluidic cell was studied. Local pH changes, induced by
                      a light-addressable electrode (LAE), were detected using a
                      light-addressable potentiometric sensor (LAPS) with
                      different measurement modes representing an actuator-sensor
                      system. Biosensor functionality was examined depending on
                      locally induced pH gradients with the help of the model
                      enzyme penicillinase, which had been immobilized in the
                      microfluidic channel. The surface morphology of the LAE and
                      enzyme-functionalized LAPS was studied by scanning electron
                      microscopy. Furthermore, the penicillin sensitivity of the
                      LAPS inside the microfluidic channel was determined with
                      regard to the analyte’s pH influence on the enzymatic
                      reaction rate. In a final experiment, the LAE-controlled pH
                      inhibition of the enzyme activity was monitored by the LAPS},
      cin          = {IBI-3},
      ddc          = {570},
      cid          = {I:(DE-Juel1)IBI-3-20200312},
      pnm          = {5241 - Molecular Information Processing in Cellular Systems
                      (POF4-524)},
      pid          = {G:(DE-HGF)POF4-5241},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {34072213},
      UT           = {WOS:000665493000001},
      doi          = {10.3390/bios11060171},
      url          = {https://juser.fz-juelich.de/record/904342},
}