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000904345 1001_ $$0P:(DE-HGF)0$$aÖzsoylu, Dua$$b0
000904345 245__ $$aCryopreservation of a cell-based biosensor chip modified with elastic polymer fibers enabling ready-to-use on-site applications
000904345 260__ $$aAmsterdam [u.a.]$$bElsevier Science$$c2021
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000904345 520__ $$aAn efficient preservation of a cell-based biosensor chip to achieve a ready-to-use on-site system is still very challenging as the chip contains a living component such as adherent mammalian cells. Herein, we propose a strategy called on-sensor cryopreservation (OSC), which enables the adherent cells to be preserved by freezing (−80 °C) on a biosensor surface, such as the light-addressable potentiometric sensor (LAPS). Adherent cells on rigid surfaces are prone to cryo-injury; thus, the surface was modified to enhance the cell recovery for OSC. It relies on i) the integration of elastic electrospun fibers composed of polyethylene vinyl acetate (PEVA), which has a high thermal expansion coefficient and low glass-transition temperature, and ii) the treatment with O2 plasma. The modified sensor is integrated into a microfluidic chip system not only to decrease the thermal mass, which is critical for fast thawing, but also to provide a precisely controlled micro-environment. This novel cryo-chip system is effective for keeping cells viable during OSC. As a proof-of-concept for the applicability of a ready-to-use format, the extracellular acidification of cancer cells (CHO-K1) was evaluated by differential LAPS measurements after thawing. Results show, for the first time, that the OSC strategy using the cryo-chip allows label-free and quantitative measurements directly after thawing, which eliminates additional post-thaw culturing steps. The freezing of the chips containing cells at the manufacturing stage and sending them via a cold-chain transport could open up a new possibility for a ready-to-use on-site system.
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000904345 7001_ $$0P:(DE-HGF)0$$aIsık, Tuğba$$b1
000904345 7001_ $$0P:(DE-HGF)0$$aDemir, Mustafa M.$$b2
000904345 7001_ $$0P:(DE-Juel1)128727$$aSchöning, Michael J.$$b3$$eCorresponding author
000904345 7001_ $$0P:(DE-HGF)0$$aWagner, Torsten$$b4
000904345 773__ $$0PERI:(DE-600)1496379-6$$a10.1016/j.bios.2021.112983$$gVol. 177, p. 112983 -$$p112983 -$$tBiosensors and bioelectronics$$v177$$x0956-5663$$y2021
000904345 8564_ $$uhttps://juser.fz-juelich.de/record/904345/files/2021_B%26B_CryoLAPS_Dua.pdf$$yRestricted
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