TY  - JOUR
AU  - Özsoylu, Dua
AU  - Isık, Tuğba
AU  - Demir, Mustafa M.
AU  - Schöning, Michael J.
AU  - Wagner, Torsten
TI  - Cryopreservation of a cell-based biosensor chip modified with elastic polymer fibers enabling ready-to-use on-site applications
JO  - Biosensors and bioelectronics
VL  - 177
SN  - 0956-5663
CY  - Amsterdam [u.a.]
PB  - Elsevier Science
M1  - FZJ-2021-05915
SP  - 112983 -
PY  - 2021
AB  - An efficient preservation of a cell-based biosensor chip to achieve a ready-to-use on-site system is still very challenging as the chip contains a living component such as adherent mammalian cells. Herein, we propose a strategy called on-sensor cryopreservation (OSC), which enables the adherent cells to be preserved by freezing (−80 °C) on a biosensor surface, such as the light-addressable potentiometric sensor (LAPS). Adherent cells on rigid surfaces are prone to cryo-injury; thus, the surface was modified to enhance the cell recovery for OSC. It relies on i) the integration of elastic electrospun fibers composed of polyethylene vinyl acetate (PEVA), which has a high thermal expansion coefficient and low glass-transition temperature, and ii) the treatment with O2 plasma. The modified sensor is integrated into a microfluidic chip system not only to decrease the thermal mass, which is critical for fast thawing, but also to provide a precisely controlled micro-environment. This novel cryo-chip system is effective for keeping cells viable during OSC. As a proof-of-concept for the applicability of a ready-to-use format, the extracellular acidification of cancer cells (CHO-K1) was evaluated by differential LAPS measurements after thawing. Results show, for the first time, that the OSC strategy using the cryo-chip allows label-free and quantitative measurements directly after thawing, which eliminates additional post-thaw culturing steps. The freezing of the chips containing cells at the manufacturing stage and sending them via a cold-chain transport could open up a new possibility for a ready-to-use on-site system.
LB  - PUB:(DE-HGF)16
C6  - 33535119
UR  - <Go to ISI:>//WOS:000618826300006
DO  - DOI:10.1016/j.bios.2021.112983
UR  - https://juser.fz-juelich.de/record/904345
ER  -