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@ARTICLE{zsoylu:904345,
      author       = {Özsoylu, Dua and Isık, Tuğba and Demir, Mustafa M. and
                      Schöning, Michael J. and Wagner, Torsten},
      title        = {{C}ryopreservation of a cell-based biosensor chip modified
                      with elastic polymer fibers enabling ready-to-use on-site
                      applications},
      journal      = {Biosensors and bioelectronics},
      volume       = {177},
      issn         = {0956-5663},
      address      = {Amsterdam [u.a.]},
      publisher    = {Elsevier Science},
      reportid     = {FZJ-2021-05915},
      pages        = {112983 -},
      year         = {2021},
      abstract     = {An efficient preservation of a cell-based biosensor chip to
                      achieve a ready-to-use on-site system is still very
                      challenging as the chip contains a living component such as
                      adherent mammalian cells. Herein, we propose a strategy
                      called on-sensor cryopreservation (OSC), which enables the
                      adherent cells to be preserved by freezing (−80 °C) on a
                      biosensor surface, such as the light-addressable
                      potentiometric sensor (LAPS). Adherent cells on rigid
                      surfaces are prone to cryo-injury; thus, the surface was
                      modified to enhance the cell recovery for OSC. It relies on
                      i) the integration of elastic electrospun fibers composed of
                      polyethylene vinyl acetate (PEVA), which has a high thermal
                      expansion coefficient and low glass-transition temperature,
                      and ii) the treatment with O2 plasma. The modified sensor is
                      integrated into a microfluidic chip system not only to
                      decrease the thermal mass, which is critical for fast
                      thawing, but also to provide a precisely controlled
                      micro-environment. This novel cryo-chip system is effective
                      for keeping cells viable during OSC. As a proof-of-concept
                      for the applicability of a ready-to-use format, the
                      extracellular acidification of cancer cells (CHO-K1) was
                      evaluated by differential LAPS measurements after thawing.
                      Results show, for the first time, that the OSC strategy
                      using the cryo-chip allows label-free and quantitative
                      measurements directly after thawing, which eliminates
                      additional post-thaw culturing steps. The freezing of the
                      chips containing cells at the manufacturing stage and
                      sending them via a cold-chain transport could open up a new
                      possibility for a ready-to-use on-site system.},
      cin          = {IBI-3},
      ddc          = {610},
      cid          = {I:(DE-Juel1)IBI-3-20200312},
      pnm          = {5241 - Molecular Information Processing in Cellular Systems
                      (POF4-524)},
      pid          = {G:(DE-HGF)POF4-5241},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {33535119},
      UT           = {WOS:000618826300006},
      doi          = {10.1016/j.bios.2021.112983},
      url          = {https://juser.fz-juelich.de/record/904345},
}