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| Hauptseite > Publikationsdatenbank > Cryopreservation of a cell-based biosensor chip modified with elastic polymer fibers enabling ready-to-use on-site applications > print |
| Hauptseite > Publikationsdatenbank > Cryopreservation of a cell-based biosensor chip modified with elastic polymer fibers enabling ready-to-use on-site applications > print |
| 001 | 904345 | ||
| 005 | 20230502100730.0 | ||
| 024 | 7 | _ | |a 10.1016/j.bios.2021.112983 |2 doi |
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| 100 | 1 | _ | |a Özsoylu, Dua |0 P:(DE-HGF)0 |b 0 |
| 245 | _ | _ | |a Cryopreservation of a cell-based biosensor chip modified with elastic polymer fibers enabling ready-to-use on-site applications |
| 260 | _ | _ | |a Amsterdam [u.a.] |c 2021 |b Elsevier Science |
| 336 | 7 | _ | |a article |2 DRIVER |
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| 336 | 7 | _ | |a Journal Article |0 0 |2 EndNote |
| 520 | _ | _ | |a An efficient preservation of a cell-based biosensor chip to achieve a ready-to-use on-site system is still very challenging as the chip contains a living component such as adherent mammalian cells. Herein, we propose a strategy called on-sensor cryopreservation (OSC), which enables the adherent cells to be preserved by freezing (−80 °C) on a biosensor surface, such as the light-addressable potentiometric sensor (LAPS). Adherent cells on rigid surfaces are prone to cryo-injury; thus, the surface was modified to enhance the cell recovery for OSC. It relies on i) the integration of elastic electrospun fibers composed of polyethylene vinyl acetate (PEVA), which has a high thermal expansion coefficient and low glass-transition temperature, and ii) the treatment with O2 plasma. The modified sensor is integrated into a microfluidic chip system not only to decrease the thermal mass, which is critical for fast thawing, but also to provide a precisely controlled micro-environment. This novel cryo-chip system is effective for keeping cells viable during OSC. As a proof-of-concept for the applicability of a ready-to-use format, the extracellular acidification of cancer cells (CHO-K1) was evaluated by differential LAPS measurements after thawing. Results show, for the first time, that the OSC strategy using the cryo-chip allows label-free and quantitative measurements directly after thawing, which eliminates additional post-thaw culturing steps. The freezing of the chips containing cells at the manufacturing stage and sending them via a cold-chain transport could open up a new possibility for a ready-to-use on-site system. |
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| 588 | _ | _ | |a Dataset connected to CrossRef, Journals: juser.fz-juelich.de |
| 700 | 1 | _ | |a Isık, Tuğba |0 P:(DE-HGF)0 |b 1 |
| 700 | 1 | _ | |a Demir, Mustafa M. |0 P:(DE-HGF)0 |b 2 |
| 700 | 1 | _ | |a Schöning, Michael J. |0 P:(DE-Juel1)128727 |b 3 |e Corresponding author |
| 700 | 1 | _ | |a Wagner, Torsten |0 P:(DE-HGF)0 |b 4 |
| 773 | _ | _ | |a 10.1016/j.bios.2021.112983 |g Vol. 177, p. 112983 - |0 PERI:(DE-600)1496379-6 |p 112983 - |t Biosensors and bioelectronics |v 177 |y 2021 |x 0956-5663 |
| 856 | 4 | _ | |u https://juser.fz-juelich.de/record/904345/files/2021_B%26B_CryoLAPS_Dua.pdf |y Restricted |
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