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@ARTICLE{FigueroaMiranda:904346,
author = {Figueroa-Miranda, Gabriela and Chen, Song and Neis, Marc
and Zhou, Lei and Zhang, Yuting and Lo, Young and Tanner,
Julian Alexander and Kreidenweiss, Andrea and Offenhäusser,
Andreas and Mayer, Dirk},
title = {{M}ulti-target electrochemical malaria aptasensor on
flexible multielectrode arrays for detection in malaria
parasite blood samples},
journal = {Sensors and actuators / B},
volume = {349},
issn = {0925-4005},
address = {Amsterdam [u.a.]},
publisher = {Elsevier Science},
reportid = {FZJ-2021-05916},
pages = {130812 -},
year = {2021},
abstract = {Despite all efforts, malaria is still highly prevalent in
tropical and developing countries. The “test, treat and
track” policy of the World Health Organization (WHO)
demands the development of affordable and highly sensitive
malaria tests that discriminate between the two common
malaria parasites, Plasmodium falciparum and Plasmodium
vivax, to guide appropriate treatments. In response, we
developed a flexible and disposable multielectrode array
utilized as electrochemical malaria aptasensor. This
multi-target aptasensor was modified by four different
aptamer receptors, discriminating between P. falciparum and
P. vivax infections via Plasmodium lactate dehydrogenase,
expressed by both species, and histidine-rich protein 2,
exclusively expressed by P. falciparum. The multi-target
aptasensor detection was tested in blood samples spiked with
target proteins, whole human blood spiked with P.
falciparum, and P. falciparum in vitro cultures. A rigorous
analysis revealed sensitivities of $>75.0\%$ for $0.001\%$
parasitemia (50 parasites/µL) and a logic gate-based
discrimination of Plasmodium infections, overcoming WHO
standards. A cost analysis further substantiated the
applicability of this multi-target aptasensor as a
disposable point-of-care test for remote areas dealing with
prevalent malaria parasite infections.},
cin = {IBI-3},
ddc = {620},
cid = {I:(DE-Juel1)IBI-3-20200312},
pnm = {5241 - Molecular Information Processing in Cellular Systems
(POF4-524)},
pid = {G:(DE-HGF)POF4-5241},
typ = {PUB:(DE-HGF)16},
UT = {WOS:000707315100008},
doi = {10.1016/j.snb.2021.130812},
url = {https://juser.fz-juelich.de/record/904346},
}