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000904349 037__ $$aFZJ-2021-05919
000904349 1001_ $$0P:(DE-HGF)0$$aJablonski, Melanie$$b0
000904349 245__ $$aDetection of plant virus particles with a capacitive field-effect sensor
000904349 260__ $$aHeidelberg$$bSpringer$$c2021
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000904349 520__ $$aPlant viruses are major contributors to crop losses and induce high economic costs worldwide. For reliable, on-site and early detection of plant viral diseases, portable biosensors are of great interest. In this study, a field-effect SiO2-gate electrolyte-insulator-semiconductor (EIS) sensor was utilized for the label-free electrostatic detection of tobacco mosaic virus (TMV) particles as a model plant pathogen. The capacitive EIS sensor has been characterized regarding its TMV sensitivity by means of constant-capacitance method. The EIS sensor was able to detect biotinylated TMV particles from a solution with a TMV concentration as low as 0.025 nM. A good correlation between the registered EIS sensor signal and the density of adsorbed TMV particles assessed from scanning electron microscopy images of the SiO2-gate chip surface was observed. Additionally, the isoelectric point of the biotinylated TMV particles was determined via zeta potential measurements and the influence of ionic strength of the measurement solution on the TMV-modified EIS sensor signal has been studied.
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000904349 7001_ $$0P:(DE-Juel1)128717$$aPoghossian, Arshak$$b1
000904349 7001_ $$0P:(DE-HGF)0$$aKeusgen, Michael$$b2
000904349 7001_ $$0P:(DE-HGF)0$$aWege, Christina$$b3
000904349 7001_ $$0P:(DE-Juel1)128727$$aSchöning, Michael J.$$b4$$eCorresponding author
000904349 773__ $$0PERI:(DE-600)1459122-4$$a10.1007/s00216-021-03448-8$$gVol. 413, no. 22, p. 5669 - 5678$$n22$$p5669 - 5678$$tAnalytical and bioanalytical chemistry$$v413$$x0016-1152$$y2021
000904349 8564_ $$uhttps://juser.fz-juelich.de/record/904349/files/Jablonski2021_Article_DetectionOfPlantVirusParticles.pdf$$yOpenAccess
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