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@ARTICLE{Jablonski:904349,
author = {Jablonski, Melanie and Poghossian, Arshak and Keusgen,
Michael and Wege, Christina and Schöning, Michael J.},
title = {{D}etection of plant virus particles with a capacitive
field-effect sensor},
journal = {Analytical and bioanalytical chemistry},
volume = {413},
number = {22},
issn = {0016-1152},
address = {Heidelberg},
publisher = {Springer},
reportid = {FZJ-2021-05919},
pages = {5669 - 5678},
year = {2021},
abstract = {Plant viruses are major contributors to crop losses and
induce high economic costs worldwide. For reliable, on-site
and early detection of plant viral diseases, portable
biosensors are of great interest. In this study, a
field-effect SiO2-gate electrolyte-insulator-semiconductor
(EIS) sensor was utilized for the label-free electrostatic
detection of tobacco mosaic virus (TMV) particles as a model
plant pathogen. The capacitive EIS sensor has been
characterized regarding its TMV sensitivity by means of
constant-capacitance method. The EIS sensor was able to
detect biotinylated TMV particles from a solution with a TMV
concentration as low as 0.025 nM. A good correlation between
the registered EIS sensor signal and the density of adsorbed
TMV particles assessed from scanning electron microscopy
images of the SiO2-gate chip surface was observed.
Additionally, the isoelectric point of the biotinylated TMV
particles was determined via zeta potential measurements and
the influence of ionic strength of the measurement solution
on the TMV-modified EIS sensor signal has been studied.},
cin = {IBI-3},
cid = {I:(DE-Juel1)IBI-3-20200312},
pnm = {5241 - Molecular Information Processing in Cellular Systems
(POF4-524)},
pid = {G:(DE-HGF)POF4-5241},
typ = {PUB:(DE-HGF)16},
pubmed = {34244834},
UT = {WOS:000671514500002},
doi = {10.1007/s00216-021-03448-8},
url = {https://juser.fz-juelich.de/record/904349},
}