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@ARTICLE{Lo:904351,
      author       = {Lo, Young and Cheung, Yee-Wai and Wang, Lin and Lee, Megan
                      and Figueroa-Miranda, Gabriela and Liang, Shaolin and Mayer,
                      Dirk and Tanner, Julian Alexander},
      title        = {{A}n electrochemical aptamer-based biosensor targeting
                      {P}lasmodium falciparum histidine-rich protein {II} for
                      malaria diagnosis},
      journal      = {Biosensors and bioelectronics},
      volume       = {192},
      issn         = {0956-5663},
      address      = {Amsterdam [u.a.]},
      publisher    = {Elsevier Science},
      reportid     = {FZJ-2021-05921},
      pages        = {113472 -},
      year         = {2021},
      abstract     = {Malaria is an infectious disease caused by parasitic
                      protozoans from the genus Plasmodium, with the species P.
                      falciparum causing the highest number of deaths worldwide.
                      Rapid diagnostic tests (RDTs) have become critical in the
                      management of malaria, but current RDTs that detect P.
                      falciparum are primarily antibody-based, which can have
                      drawbacks in cost and robustness. Here, we report the
                      development of an electrochemical aptamer-based (E-AB)
                      biosensing alternative. Through selective evolution of
                      ligands by exponential enrichment, we identify DNA aptamers
                      that bind specifically to P. falciparum histidine-rich
                      protein II (PfHRP2). The aptamer is modified with a
                      methylene blue reporter and attached to a gold sensor
                      surface for square-wave voltammetry interrogation. Through
                      this method we are able to quantify PfHRP2 in human serum
                      with an LOD of 3.73 nM. We further demonstrate the biosensor
                      is stable in serum buffers and reusable for multiple
                      detection rounds. These findings provide a promising
                      alternative to conventional PfHRP2 detection for malaria
                      diagnosis, while also expanding the capabilities of E-AB
                      biosensors.},
      cin          = {IBI-3},
      ddc          = {610},
      cid          = {I:(DE-Juel1)IBI-3-20200312},
      pnm          = {5241 - Molecular Information Processing in Cellular Systems
                      (POF4-524)},
      pid          = {G:(DE-HGF)POF4-5241},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {34271397},
      UT           = {WOS:000704056300009},
      doi          = {10.1016/j.bios.2021.113472},
      url          = {https://juser.fz-juelich.de/record/904351},
}