%0 Journal Article
%A Minopoli, Antonio
%A Della Ventura, Bartolomeo
%A Campanile, Raffaele
%A Tanner, Julian A.
%A Offenhäusser, Andreas
%A Mayer, Dirk
%A Velotta, Raffaele
%T Randomly positioned gold nanoparticles as fluorescence enhancers in apta-immunosensor for malaria test
%J Microchimica acta
%V 188
%N 3
%@ 0026-3672
%C Wien [u.a.]
%I Springer
%M FZJ-2021-05923
%P 88
%D 2021
%X A plasmon-enhanced fluorescence-based antibody-aptamer biosensor — consisting of gold nanoparticles randomly immobilized onto a glass substrate via electrostatic self-assembly — is described for specific detection of proteins in whole blood. Analyte recognition is realized through a sandwich scheme with a capture bioreceptor layer of antibodies — covalently immobilized onto the gold nanoparticle surface in upright orientation and close-packed configuration by photochemical immobilization technique (PIT) — and a top bioreceptor layer of fluorescently labelled aptamers. Such a sandwich configuration warrants not only extremely high specificity, but also an ideal fluorophore-nanostructure distance (approximately 10–15 nm) for achieving strong fluorescence amplification. For a specific application, we tested the biosensor performance in a case study for the detection of malaria-related marker Plasmodium falciparum lactate dehydrogenase (PfLDH). The proposed biosensor can specifically detect PfLDH in spiked whole blood down to 10 pM (0.3 ng/mL) without any sample pretreatment. The combination of simple and scalable fabrication, potentially high-throughput analysis, and excellent sensing performance provides a new approach to biosensing with significant advantages compared to conventional fluorescence immunoassays
%F PUB:(DE-HGF)16
%9 Journal Article
%$ 33594523
%U <Go to ISI:>//WOS:000620547300001
%R 10.1007/s00604-021-04746-9
%U https://juser.fz-juelich.de/record/904353