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000904353 1001_ $$0P:(DE-HGF)0$$aMinopoli, Antonio$$b0
000904353 245__ $$aRandomly positioned gold nanoparticles as fluorescence enhancers in apta-immunosensor for malaria test
000904353 260__ $$aWien [u.a.]$$bSpringer$$c2021
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000904353 520__ $$aA plasmon-enhanced fluorescence-based antibody-aptamer biosensor — consisting of gold nanoparticles randomly immobilized onto a glass substrate via electrostatic self-assembly — is described for specific detection of proteins in whole blood. Analyte recognition is realized through a sandwich scheme with a capture bioreceptor layer of antibodies — covalently immobilized onto the gold nanoparticle surface in upright orientation and close-packed configuration by photochemical immobilization technique (PIT) — and a top bioreceptor layer of fluorescently labelled aptamers. Such a sandwich configuration warrants not only extremely high specificity, but also an ideal fluorophore-nanostructure distance (approximately 10–15 nm) for achieving strong fluorescence amplification. For a specific application, we tested the biosensor performance in a case study for the detection of malaria-related marker Plasmodium falciparum lactate dehydrogenase (PfLDH). The proposed biosensor can specifically detect PfLDH in spiked whole blood down to 10 pM (0.3 ng/mL) without any sample pretreatment. The combination of simple and scalable fabrication, potentially high-throughput analysis, and excellent sensing performance provides a new approach to biosensing with significant advantages compared to conventional fluorescence immunoassays
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000904353 7001_ $$0P:(DE-HGF)0$$aDella Ventura, Bartolomeo$$b1
000904353 7001_ $$0P:(DE-HGF)0$$aCampanile, Raffaele$$b2
000904353 7001_ $$0P:(DE-HGF)0$$aTanner, Julian A.$$b3
000904353 7001_ $$0P:(DE-Juel1)128713$$aOffenhäusser, Andreas$$b4
000904353 7001_ $$0P:(DE-Juel1)128707$$aMayer, Dirk$$b5$$eCorresponding author
000904353 7001_ $$00000-0003-1077-8353$$aVelotta, Raffaele$$b6
000904353 773__ $$0PERI:(DE-600)1462152-6$$a10.1007/s00604-021-04746-9$$gVol. 188, no. 3, p. 88$$n3$$p88$$tMicrochimica acta$$v188$$x0026-3672$$y2021
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