TY  - JOUR
AU  - Minopoli, Antonio
AU  - Della Ventura, Bartolomeo
AU  - Campanile, Raffaele
AU  - Tanner, Julian A.
AU  - Offenhäusser, Andreas
AU  - Mayer, Dirk
AU  - Velotta, Raffaele
TI  - Randomly positioned gold nanoparticles as fluorescence enhancers in apta-immunosensor for malaria test
JO  - Microchimica acta
VL  - 188
IS  - 3
SN  - 0026-3672
CY  - Wien [u.a.]
PB  - Springer
M1  - FZJ-2021-05923
SP  - 88
PY  - 2021
AB  - A plasmon-enhanced fluorescence-based antibody-aptamer biosensor — consisting of gold nanoparticles randomly immobilized onto a glass substrate via electrostatic self-assembly — is described for specific detection of proteins in whole blood. Analyte recognition is realized through a sandwich scheme with a capture bioreceptor layer of antibodies — covalently immobilized onto the gold nanoparticle surface in upright orientation and close-packed configuration by photochemical immobilization technique (PIT) — and a top bioreceptor layer of fluorescently labelled aptamers. Such a sandwich configuration warrants not only extremely high specificity, but also an ideal fluorophore-nanostructure distance (approximately 10–15 nm) for achieving strong fluorescence amplification. For a specific application, we tested the biosensor performance in a case study for the detection of malaria-related marker Plasmodium falciparum lactate dehydrogenase (PfLDH). The proposed biosensor can specifically detect PfLDH in spiked whole blood down to 10 pM (0.3 ng/mL) without any sample pretreatment. The combination of simple and scalable fabrication, potentially high-throughput analysis, and excellent sensing performance provides a new approach to biosensing with significant advantages compared to conventional fluorescence immunoassays
LB  - PUB:(DE-HGF)16
C6  - 33594523
UR  - <Go to ISI:>//WOS:000620547300001
DO  - DOI:10.1007/s00604-021-04746-9
UR  - https://juser.fz-juelich.de/record/904353
ER  -