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@ARTICLE{Minopoli:904353,
      author       = {Minopoli, Antonio and Della Ventura, Bartolomeo and
                      Campanile, Raffaele and Tanner, Julian A. and Offenhäusser,
                      Andreas and Mayer, Dirk and Velotta, Raffaele},
      title        = {{R}andomly positioned gold nanoparticles as fluorescence
                      enhancers in apta-immunosensor for malaria test},
      journal      = {Microchimica acta},
      volume       = {188},
      number       = {3},
      issn         = {0026-3672},
      address      = {Wien [u.a.]},
      publisher    = {Springer},
      reportid     = {FZJ-2021-05923},
      pages        = {88},
      year         = {2021},
      abstract     = {A plasmon-enhanced fluorescence-based antibody-aptamer
                      biosensor — consisting of gold nanoparticles randomly
                      immobilized onto a glass substrate via electrostatic
                      self-assembly — is described for specific detection of
                      proteins in whole blood. Analyte recognition is realized
                      through a sandwich scheme with a capture bioreceptor layer
                      of antibodies — covalently immobilized onto the gold
                      nanoparticle surface in upright orientation and close-packed
                      configuration by photochemical immobilization technique
                      (PIT) — and a top bioreceptor layer of fluorescently
                      labelled aptamers. Such a sandwich configuration warrants
                      not only extremely high specificity, but also an ideal
                      fluorophore-nanostructure distance (approximately 10–15
                      nm) for achieving strong fluorescence amplification. For a
                      specific application, we tested the biosensor performance in
                      a case study for the detection of malaria-related marker
                      Plasmodium falciparum lactate dehydrogenase (PfLDH). The
                      proposed biosensor can specifically detect PfLDH in spiked
                      whole blood down to 10 pM (0.3 ng/mL) without any sample
                      pretreatment. The combination of simple and scalable
                      fabrication, potentially high-throughput analysis, and
                      excellent sensing performance provides a new approach to
                      biosensing with significant advantages compared to
                      conventional fluorescence immunoassays},
      cin          = {IBI-3},
      ddc          = {540},
      cid          = {I:(DE-Juel1)IBI-3-20200312},
      pnm          = {5241 - Molecular Information Processing in Cellular Systems
                      (POF4-524)},
      pid          = {G:(DE-HGF)POF4-5241},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {33594523},
      UT           = {WOS:000620547300001},
      doi          = {10.1007/s00604-021-04746-9},
      url          = {https://juser.fz-juelich.de/record/904353},
}