Home > Publications database > Randomly positioned gold nanoparticles as fluorescence enhancers in apta-immunosensor for malaria test > print

001     904353
005     20230123101846.0
024 7 _ |a 10.1007/s00604-021-04746-9
|2 doi
024 7 _ |a 0026-3672
|2 ISSN
024 7 _ |a 0344-838X
|2 ISSN
024 7 _ |a 0344-8436
|2 ISSN
024 7 _ |a 0369-0504
|2 ISSN
024 7 _ |a 0369-2795
|2 ISSN
024 7 _ |a 1436-5073
|2 ISSN
024 7 _ |a 2128/33175
|2 Handle
024 7 _ |a 33594523
|2 pmid
024 7 _ |a WOS:000620547300001
|2 WOS
037 _ _ |a FZJ-2021-05923
082 _ _ |a 540
100 1 _ |a Minopoli, Antonio
|0 P:(DE-HGF)0
|b 0
245 _ _ |a Randomly positioned gold nanoparticles as fluorescence enhancers in apta-immunosensor for malaria test
260 _ _ |a Wien [u.a.]
|c 2021
|b Springer
336 7 _ |a article
|2 DRIVER
336 7 _ |a Output Types/Journal article
|2 DataCite
336 7 _ |a Journal Article
|b journal
|m journal
|0 PUB:(DE-HGF)16
|s 1671199684_4882
|2 PUB:(DE-HGF)
336 7 _ |a ARTICLE
|2 BibTeX
336 7 _ |a JOURNAL_ARTICLE
|2 ORCID
336 7 _ |a Journal Article
|0 0
|2 EndNote
520 _ _ |a A plasmon-enhanced fluorescence-based antibody-aptamer biosensor — consisting of gold nanoparticles randomly immobilized onto a glass substrate via electrostatic self-assembly — is described for specific detection of proteins in whole blood. Analyte recognition is realized through a sandwich scheme with a capture bioreceptor layer of antibodies — covalently immobilized onto the gold nanoparticle surface in upright orientation and close-packed configuration by photochemical immobilization technique (PIT) — and a top bioreceptor layer of fluorescently labelled aptamers. Such a sandwich configuration warrants not only extremely high specificity, but also an ideal fluorophore-nanostructure distance (approximately 10–15 nm) for achieving strong fluorescence amplification. For a specific application, we tested the biosensor performance in a case study for the detection of malaria-related marker Plasmodium falciparum lactate dehydrogenase (PfLDH). The proposed biosensor can specifically detect PfLDH in spiked whole blood down to 10 pM (0.3 ng/mL) without any sample pretreatment. The combination of simple and scalable fabrication, potentially high-throughput analysis, and excellent sensing performance provides a new approach to biosensing with significant advantages compared to conventional fluorescence immunoassays
536 _ _ |a 5241 - Molecular Information Processing in Cellular Systems (POF4-524)
|0 G:(DE-HGF)POF4-5241
|c POF4-524
|f POF IV
|x 0
588 _ _ |a Dataset connected to CrossRef, Journals: juser.fz-juelich.de
700 1 _ |a Della Ventura, Bartolomeo
|0 P:(DE-HGF)0
|b 1
700 1 _ |a Campanile, Raffaele
|0 P:(DE-HGF)0
|b 2
700 1 _ |a Tanner, Julian A.
|0 P:(DE-HGF)0
|b 3
700 1 _ |a Offenhäusser, Andreas
|0 P:(DE-Juel1)128713
|b 4
700 1 _ |a Mayer, Dirk
|0 P:(DE-Juel1)128707
|b 5
|e Corresponding author
700 1 _ |a Velotta, Raffaele
|0 0000-0003-1077-8353
|b 6
773 _ _ |a 10.1007/s00604-021-04746-9
|g Vol. 188, no. 3, p. 88
|0 PERI:(DE-600)1462152-6
|n 3
|p 88
|t Microchimica acta
|v 188
|y 2021
|x 0026-3672
856 4 _ |u https://juser.fz-juelich.de/record/904353/files/Minopoli2021_Article_RandomlyPositionedGoldNanopart.pdf
|y OpenAccess
909 C O |o oai:juser.fz-juelich.de:904353
|p openaire
|p open_access
|p VDB
|p driver
|p dnbdelivery
910 1 _ |a Forschungszentrum Jülich
|0 I:(DE-588b)5008462-8
|k FZJ
|b 4
|6 P:(DE-Juel1)128713
910 1 _ |a Forschungszentrum Jülich
|0 I:(DE-588b)5008462-8
|k FZJ
|b 5
|6 P:(DE-Juel1)128707
910 1 _ |a External Institute
|0 I:(DE-HGF)0
|k Extern
|b 6
|6 0000-0003-1077-8353
913 1 _ |a DE-HGF
|b Key Technologies
|l Natural, Artificial and Cognitive Information Processing
|1 G:(DE-HGF)POF4-520
|0 G:(DE-HGF)POF4-524
|3 G:(DE-HGF)POF4
|2 G:(DE-HGF)POF4-500
|4 G:(DE-HGF)POF
|v Molecular and Cellular Information Processing
|9 G:(DE-HGF)POF4-5241
|x 0
914 1 _ |y 2022
915 _ _ |a DBCoverage
|0 StatID:(DE-HGF)0200
|2 StatID
|b SCOPUS
|d 2021-01-27
915 _ _ |a DBCoverage
|0 StatID:(DE-HGF)0160
|2 StatID
|b Essential Science Indicators
|d 2021-01-27
915 _ _ |a Creative Commons Attribution CC BY 4.0
|0 LIC:(DE-HGF)CCBY4
|2 HGFVOC
915 _ _ |a DBCoverage
|0 StatID:(DE-HGF)0600
|2 StatID
|b Ebsco Academic Search
|d 2021-01-27
915 _ _ |a DBCoverage
|0 StatID:(DE-HGF)1150
|2 StatID
|b Current Contents - Physical, Chemical and Earth Sciences
|d 2021-01-27
915 _ _ |a IF >= 5
|0 StatID:(DE-HGF)9905
|2 StatID
|b MICROCHIM ACTA : 2019
|d 2021-01-27
915 _ _ |a WoS
|0 StatID:(DE-HGF)0113
|2 StatID
|b Science Citation Index Expanded
|d 2021-01-27
915 _ _ |a DEAL Springer
|0 StatID:(DE-HGF)3002
|2 StatID
|d 2021-01-27
|w ger
915 _ _ |a DBCoverage
|0 StatID:(DE-HGF)0150
|2 StatID
|b Web of Science Core Collection
|d 2021-01-27
915 _ _ |a OpenAccess
|0 StatID:(DE-HGF)0510
|2 StatID
915 _ _ |a Peer Review
|0 StatID:(DE-HGF)0030
|2 StatID
|b ASC
|d 2021-01-27
915 _ _ |a JCR
|0 StatID:(DE-HGF)0100
|2 StatID
|b MICROCHIM ACTA : 2019
|d 2021-01-27
915 _ _ |a DBCoverage
|0 StatID:(DE-HGF)0300
|2 StatID
|b Medline
|d 2021-01-27
915 _ _ |a Nationallizenz
|0 StatID:(DE-HGF)0420
|2 StatID
|d 2021-01-27
|w ger
915 _ _ |a DBCoverage
|0 StatID:(DE-HGF)0199
|2 StatID
|b Clarivate Analytics Master Journal List
|d 2021-01-27
920 1 _ |0 I:(DE-Juel1)IBI-3-20200312
|k IBI-3
|l Bioelektronik
|x 0
980 1 _ |a FullTexts
980 _ _ |a journal
980 _ _ |a VDB
980 _ _ |a UNRESTRICTED
980 _ _ |a I:(DE-Juel1)IBI-3-20200312

LibraryCollectionCLSMajorCLSMinorLanguageAuthor
Marc 21