TY  - JOUR
AU  - Oliveira, Danilo A.
AU  - Molinnus, Denise
AU  - Beging, Stefan
AU  - Siqueira, José R.
AU  - Schöning, Michael J.
TI  - Biosensor Based on Self‐Assembled Films of Graphene Oxide and Polyaniline Using a Field‐Effect Device Platform
JO  - Physica status solidi / A
VL  - 218
IS  - 13
SN  - 0031-8965
CY  - Weinheim
PB  - Wiley-VCH
M1  - FZJ-2021-05926
SP  - 2000747 -
PY  - 2021
AB  - A new functionalization method to modify capacitive electrolyte–insulator–semiconductor (EIS) structures with nanofilms is presented. Layers of polyallylamine hydrochloride (PAH) and graphene oxide (GO) with the compound polyaniline:poly(2-acrylamido-2-methyl-1-propanesulfonic acid) (PANI:PAAMPSA) are deposited onto a p-Si/SiO2 chip using the layer-by-layer technique (LbL). Two different enzymes (urease and penicillinase) are separately immobilized on top of a five-bilayer stack of the PAH:GO/PANI:PAAMPSA-modified EIS chip, forming a biosensor for detection of urea and penicillin, respectively. Electrochemical characterization is performed by constant capacitance (ConCap) measurements, and the film morphology is characterized by atomic force microscopy (AFM) and scanning electron microscopy (SEM). An increase in the average sensitivity of the modified biosensors (EIS–nanofilm–enzyme) of around 15% is found in relation to sensors, only carrying the enzyme but without the nanofilm (EIS–enzyme). In this sense, the nanofilm acts as a stable bioreceptor onto the EIS chip improving the output signal in terms of sensitivity and stability.
LB  - PUB:(DE-HGF)16
UR  - <Go to ISI:>//WOS:000613842900001
DO  - DOI:10.1002/pssa.202000747
UR  - https://juser.fz-juelich.de/record/904356
ER  -