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000904476 1001_ $$0P:(DE-HGF)0$$aDemir, Hatice Taşpınar$$b0
000904476 245__ $$aHigh level production of itaconic acid at low pH by Ustilago maydis with fed-batch fermentation
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000904476 520__ $$aThe metabolically engineered plant pathogen Ustilago maydis MB215 Δcyp3 Petefria1 has been cultivated to produce more than 80 g/L itaconate in 16 L scale pH and temperature controlled fermentation, in fed-batch mode with two successive feedings. The effect of pH as well as successive rounds of feeding has been quantified via elemental balances. Volumetric itaconic acid productivity gradually decreased with successive glucose feedings with increasing itaconic titers, with nearly constant product yield. Extracellular pH was decreased from 6 down to 3.5 and the fermentation was characterized in specific uptake, production, and growth rates. Notable is that the biomass composition changes significantly from growth phase to itaconic acid production phase, carbon content increases from 42% to around 62%. Despite the gradual decrease in itaconic acid levels with decreasing pH (nearly 50% decrease in itaconic acid at pH 3.5, compared to pH 6), significant itaconate production is still observed at pH 4 (around 63 g/L). Biomass yield remained nearly constant until pH 4. Taken together, these results strongly illustrate the potential of engineered Ustilago maydis in itaconate production at commercial levels.
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000904476 7001_ $$0P:(DE-HGF)0$$aBezirci, Emine$$b1
000904476 7001_ $$0P:(DE-HGF)0$$aBecker, Johanna$$b2
000904476 7001_ $$0P:(DE-HGF)0$$aTehrani, Hamed Hosseinpour$$b3
000904476 7001_ $$0P:(DE-HGF)0$$aNikerel, Emrah$$b4
000904476 7001_ $$0P:(DE-HGF)0$$aWierck, Nick$$b5
000904476 7001_ $$0P:(DE-HGF)0$$aTürker, Mustafa$$b6$$eCorresponding author
000904476 773__ $$0PERI:(DE-600)1476357-6$$a10.1007/s00449-020-02483-6$$gVol. 44, no. 4, p. 749 - 758$$n4$$p749 - 758$$tBioprocess and biosystems engineering$$v44$$x0178-515X$$y2021
000904476 8564_ $$uhttps://juser.fz-juelich.de/record/904476/files/Demir%202021%20ustilago%20fed%20batch.pdf$$yPublished on 2021-01-03. Available in OpenAccess from 2022-01-03.$$zStatID:(DE-HGF)0510
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