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@ARTICLE{Demir:904476,
      author       = {Demir, Hatice Taşpınar and Bezirci, Emine and Becker,
                      Johanna and Tehrani, Hamed Hosseinpour and Nikerel, Emrah
                      and Wierck, Nick and Türker, Mustafa},
      title        = {{H}igh level production of itaconic acid at low p{H} by
                      {U}stilago maydis with fed-batch fermentation},
      journal      = {Bioprocess and biosystems engineering},
      volume       = {44},
      number       = {4},
      issn         = {0178-515X},
      address      = {New York},
      publisher    = {Springer},
      reportid     = {FZJ-2021-06046},
      pages        = {749 - 758},
      year         = {2021},
      abstract     = {The metabolically engineered plant pathogen Ustilago maydis
                      MB215 Δcyp3 Petefria1 has been cultivated to produce more
                      than 80 g/L itaconate in 16 L scale pH and temperature
                      controlled fermentation, in fed-batch mode with two
                      successive feedings. The effect of pH as well as successive
                      rounds of feeding has been quantified via elemental
                      balances. Volumetric itaconic acid productivity gradually
                      decreased with successive glucose feedings with increasing
                      itaconic titers, with nearly constant product yield.
                      Extracellular pH was decreased from 6 down to 3.5 and the
                      fermentation was characterized in specific uptake,
                      production, and growth rates. Notable is that the biomass
                      composition changes significantly from growth phase to
                      itaconic acid production phase, carbon content increases
                      from $42\%$ to around $62\%.$ Despite the gradual decrease
                      in itaconic acid levels with decreasing pH (nearly $50\%$
                      decrease in itaconic acid at pH 3.5, compared to pH 6),
                      significant itaconate production is still observed at pH 4
                      (around 63 g/L). Biomass yield remained nearly constant
                      until pH 4. Taken together, these results strongly
                      illustrate the potential of engineered Ustilago maydis in
                      itaconate production at commercial levels.},
      cin          = {IBG-1},
      ddc          = {690},
      cid          = {I:(DE-Juel1)IBG-1-20101118},
      pnm          = {2172 - Utilization of renewable carbon and energy sources
                      and engineering of ecosystem functions (POF4-217)},
      pid          = {G:(DE-HGF)POF4-2172},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {33392747},
      UT           = {WOS:000604524100006},
      doi          = {10.1007/s00449-020-02483-6},
      url          = {https://juser.fz-juelich.de/record/904476},
}