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000904477 1001_ $$0P:(DE-Juel1)171827$$aDemling, Philipp$$b0
000904477 245__ $$aPseudomonas putida KT2440 endures temporary oxygen limitations
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000904477 520__ $$aThe obligate aerobic nature of Pseudomonas putida, one of the most prominent whole-cell biocatalysts emerging for industrial bioprocesses, questions its ability to be cultivated in large-scale bioreactors, which exhibit zones of low dissolved oxygen tension. P. putida KT2440 was repeatedly subjected to temporary oxygen limitations in scale-down approaches to assess the effect on growth and an exemplary production of rhamnolipids. At those conditions, the growth and production of P. putida KT2440 were decelerated compared to well-aerated reference cultivations, but remarkably, final biomass and rhamnolipid titers were similar. The robust growth behavior was confirmed across different cultivation systems, media compositions, and laboratories, even when P. putida KT2440 was repeatedly exposed to dual carbon and oxygen starvation. Quantification of the nucleotides ATP, ADP, and AMP revealed a decrease of intracellular ATP concentrations with increasing duration of oxygen starvation, which can, however, be restored when re-supplied with oxygen. Only small changes in the proteome were detected when cells encountered oscillations in dissolved oxygen tensions. Concluding, P. putida KT2440 appears to be able to cope with repeated oxygen limitations as they occur in large-scale bioreactors, affirming its outstanding suitability as a whole-cell biocatalyst for industrial-scale bioprocesses.
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000904477 7001_ $$00000-0002-2843-683X$$aAnkenbauer, Andreas$$b1
000904477 7001_ $$0P:(DE-Juel1)129034$$aKlein, Bianca$$b2$$ufzj
000904477 7001_ $$0P:(DE-Juel1)129050$$aNoack, Stephan$$b3
000904477 7001_ $$00000-0003-4420-5609$$aTiso, Till$$b4
000904477 7001_ $$00000-0001-5837-6906$$aTakors, Ralf$$b5
000904477 7001_ $$00000-0003-0961-4976$$aBlank, Lars M.$$b6$$eCorresponding author
000904477 773__ $$0PERI:(DE-600)1480809-2$$a10.1002/bit.27938$$gVol. 118, no. 12, p. 4735 - 4750$$n12$$p4735 - 4750$$tBiotechnology & bioengineering$$v118$$x0006-3592$$y2021
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