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@ARTICLE{Demling:904477,
      author       = {Demling, Philipp and Ankenbauer, Andreas and Klein, Bianca
                      and Noack, Stephan and Tiso, Till and Takors, Ralf and
                      Blank, Lars M.},
      title        = {{P}seudomonas putida {KT}2440 endures temporary oxygen
                      limitations},
      journal      = {Biotechnology $\&$ bioengineering},
      volume       = {118},
      number       = {12},
      issn         = {0006-3592},
      address      = {New York, NY [u.a.]},
      publisher    = {Wiley},
      reportid     = {FZJ-2021-06047},
      pages        = {4735 - 4750},
      year         = {2021},
      abstract     = {The obligate aerobic nature of Pseudomonas putida, one of
                      the most prominent whole-cell biocatalysts emerging for
                      industrial bioprocesses, questions its ability to be
                      cultivated in large-scale bioreactors, which exhibit zones
                      of low dissolved oxygen tension. P. putida KT2440 was
                      repeatedly subjected to temporary oxygen limitations in
                      scale-down approaches to assess the effect on growth and an
                      exemplary production of rhamnolipids. At those conditions,
                      the growth and production of P. putida KT2440 were
                      decelerated compared to well-aerated reference cultivations,
                      but remarkably, final biomass and rhamnolipid titers were
                      similar. The robust growth behavior was confirmed across
                      different cultivation systems, media compositions, and
                      laboratories, even when P. putida KT2440 was repeatedly
                      exposed to dual carbon and oxygen starvation. Quantification
                      of the nucleotides ATP, ADP, and AMP revealed a decrease of
                      intracellular ATP concentrations with increasing duration of
                      oxygen starvation, which can, however, be restored when
                      re-supplied with oxygen. Only small changes in the proteome
                      were detected when cells encountered oscillations in
                      dissolved oxygen tensions. Concluding, P. putida KT2440
                      appears to be able to cope with repeated oxygen limitations
                      as they occur in large-scale bioreactors, affirming its
                      outstanding suitability as a whole-cell biocatalyst for
                      industrial-scale bioprocesses.},
      cin          = {IBG-1},
      ddc          = {570},
      cid          = {I:(DE-Juel1)IBG-1-20101118},
      pnm          = {2172 - Utilization of renewable carbon and energy sources
                      and engineering of ecosystem functions (POF4-217)},
      pid          = {G:(DE-HGF)POF4-2172},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:34506651},
      UT           = {WOS:000697897700001},
      doi          = {10.1002/bit.27938},
      url          = {https://juser.fz-juelich.de/record/904477},
}