Home > Publications database > Pseudomonas putida KT2440 endures temporary oxygen limitations > print

001     904477
005     20220131120428.0
024 7 _ |a 10.1002/bit.27938
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100 1 _ |a Demling, Philipp
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245 _ _ |a Pseudomonas putida KT2440 endures temporary oxygen limitations
260 _ _ |a New York, NY [u.a.]
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520 _ _ |a The obligate aerobic nature of Pseudomonas putida, one of the most prominent whole-cell biocatalysts emerging for industrial bioprocesses, questions its ability to be cultivated in large-scale bioreactors, which exhibit zones of low dissolved oxygen tension. P. putida KT2440 was repeatedly subjected to temporary oxygen limitations in scale-down approaches to assess the effect on growth and an exemplary production of rhamnolipids. At those conditions, the growth and production of P. putida KT2440 were decelerated compared to well-aerated reference cultivations, but remarkably, final biomass and rhamnolipid titers were similar. The robust growth behavior was confirmed across different cultivation systems, media compositions, and laboratories, even when P. putida KT2440 was repeatedly exposed to dual carbon and oxygen starvation. Quantification of the nucleotides ATP, ADP, and AMP revealed a decrease of intracellular ATP concentrations with increasing duration of oxygen starvation, which can, however, be restored when re-supplied with oxygen. Only small changes in the proteome were detected when cells encountered oscillations in dissolved oxygen tensions. Concluding, P. putida KT2440 appears to be able to cope with repeated oxygen limitations as they occur in large-scale bioreactors, affirming its outstanding suitability as a whole-cell biocatalyst for industrial-scale bioprocesses.
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700 1 _ |a Ankenbauer, Andreas
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700 1 _ |a Klein, Bianca
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700 1 _ |a Noack, Stephan
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700 1 _ |a Tiso, Till
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700 1 _ |a Takors, Ralf
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700 1 _ |a Blank, Lars M.
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|e Corresponding author
773 _ _ |a 10.1002/bit.27938
|g Vol. 118, no. 12, p. 4735 - 4750
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856 4 _ |u https://juser.fz-juelich.de/record/904477/files/Biotech%20Bioengineering%20-%202021%20-%20Demling%20-%20Pseudomonas%20putida%20KT2440%20endures%20temporary%20oxygen%20limitations.pdf
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