TY  - JOUR
AU  - Nguyen, Bach-Ngan
AU  - Tieves, Florian
AU  - Rohr, Thomas
AU  - Wobst, Hilke
AU  - Schöpf, Felix S.
AU  - Solano, Jóse D. Montoya
AU  - Schneider, Julia
AU  - Stock, Janpeter
AU  - Uhde, Andreas
AU  - Kalthoff, Thomas
AU  - Jaeger, Karl Erich
AU  - Schmitt, Lutz
AU  - Schwarz, Christian
TI  - Numaswitch: an efficient high-titer expression platform to produce peptides and small proteins
JO  - AMB express
VL  - 11
IS  - 1
SN  - 2191-0855
CY  - Heidelberg
PB  - Springer
M1  - FZJ-2021-06056
SP  - 48
PY  - 2021
AB  - The production of peptides as active pharmaceutical ingredients (APIs) by recombinant technologies is of emerging interest. A reliable production platform, however, is still missing due the inherent characteristics of peptides such as proteolytic sensitivity, aggregation and cytotoxicity. We have developed a new technology named Numaswitch solving present limitations. Numaswitch was successfully employed for the production of diverse peptides and small proteins varying in length, physicochemical and functional characteristics, including Teriparatide, Linaclotide, human β-amyloid and Serum amyloid A3. Additionally, the potential of Numaswitch for a cost-efficient commercial production is demonstrated yielding > 2 g Teriparatide per liter fermentation broth in a quality meeting API standard.
LB  - PUB:(DE-HGF)16
C6  - pmid:33765268
UR  - <Go to ISI:>//WOS:000632906300001
DO  - DOI:10.1186/s13568-021-01204-w
UR  - https://juser.fz-juelich.de/record/904486
ER  -