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@ARTICLE{Chatterjee:904495,
      author       = {Chatterjee, Jolly and Coe, Robert A and Acebron, Kelvin and
                      Thakur, Vivek and Yennamalli, Ragothaman M and Danila,
                      Florence and Lin, Hsiang-Chun and Balahadia, Christian Paolo
                      and Bagunu, Efren and Padhma, Preiya P O S and Bala, Soumi
                      and Yin, Xiaojia and Rizal, Govinda and Dionora, Jacqueline
                      and Furbank, Robert T and von Caemmerer, Susanne and Quick,
                      William Paul},
      title        = {{A} low {CO}2-responsive mutant of {S}etaria viridis
                      reveals that reduced carbonic anhydrase limits {C}4
                      photosynthesis},
      journal      = {The journal of experimental botany},
      volume       = {72},
      number       = {8},
      issn         = {0022-0957},
      address      = {Oxford},
      publisher    = {Oxford Univ. Press},
      reportid     = {FZJ-2021-06065},
      pages        = {3122 - 3136},
      year         = {2021},
      abstract     = {In C4 species, β-carbonic anhydrase (CA), localized to the
                      cytosol of the mesophyll cells, accelerates the
                      interconversion of CO2 to HCO3–, the substrate used by
                      phosphoenolpyruvate carboxylase (PEPC) in the first step of
                      C4 photosynthesis. Here we describe the identification and
                      characterization of low CO2-responsive mutant 1 (lcr1)
                      isolated from an N-nitroso-N-methylurea- (NMU) treated
                      Setaria viridis mutant population. Forward genetic
                      investigation revealed that the mutated gene Sevir.5G247800
                      of lcr1 possessed a single nucleotide transition from
                      cytosine to thymine in a β-CA gene causing an amino acid
                      change from leucine to phenylalanine. This resulted in
                      severe reduction in growth and photosynthesis in the mutant.
                      Both the CO2 compensation point and carbon isotope
                      discrimination values of the mutant were significantly
                      increased. Growth of the mutants was stunted when grown
                      under ambient pCO2 but recovered at elevated pCO2. Further
                      bioinformatics analyses revealed that the mutation has led
                      to functional changes in one of the conserved residues of
                      the protein, situated near the catalytic site. CA transcript
                      accumulation in the mutant was $80\%$ lower, CA protein
                      accumulation $30\%$ lower, and CA activity $~98\%$ lower
                      compared with the wild type. Changes in the abundance of
                      other primary C4 pathway enzymes were observed; accumulation
                      of PEPC protein was significantly increased and accumulation
                      of malate dehydrogenase and malic enzyme decreased. The
                      reduction of CA protein activity and abundance in lcr1
                      restricts the supply of bicarbonate to PEPC, limiting C4
                      photosynthesis and growth. This study establishes
                      Sevir.5G247800 as the major CA allele in Setaria for C4
                      photosynthesis and provides important insights into the
                      function of CA in C4 photosynthesis that would be required
                      to generate a rice plant with a functional C4 biochemical
                      pathway.},
      cin          = {IBG-2},
      ddc          = {580},
      cid          = {I:(DE-Juel1)IBG-2-20101118},
      pnm          = {2171 - Biological and environmental resources for
                      sustainable use (POF4-217)},
      pid          = {G:(DE-HGF)POF4-2171},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:33528493},
      UT           = {WOS:000642310500024},
      doi          = {10.1093/jxb/erab039},
      url          = {https://juser.fz-juelich.de/record/904495},
}