TY  - JOUR
AU  - Moudříková, Šárka
AU  - Ivanov, Ivan Nedyalkov
AU  - Vítová, Milada
AU  - Nedbal, Ladislav
AU  - Zachleder, Vilém
AU  - Mojzeš, Peter
AU  - Bišová, Kateřina
TI  - Comparing Biochemical and Raman Microscopy Analyses of Starch, Lipids, Polyphosphate, and Guanine Pools during the Cell Cycle of Desmodesmus quadricauda
JO  - Cells
VL  - 10
IS  - 1
SN  - 2073-4409
CY  - Basel
PB  - MDPI
M1  - FZJ-2021-06077
SP  - 62 -
PY  - 2021
AB  - Photosynthetic energy conversion and the resulting photoautotrophic growth of green algae can only occur in daylight, but DNA replication, nuclear and cellular divisions occur often during the night. With such a light/dark regime, an algal culture becomes synchronized. In this study, using synchronized cultures of the green alga Desmodesmus quadricauda, the dynamics of starch, lipid, polyphosphate, and guanine pools were investigated during the cell cycle by two independent methodologies; conventional biochemical analyzes of cell suspensions and confocal Raman microscopy of single algal cells. Raman microscopy reports not only on mean concentrations, but also on the distribution of pools within cells. This is more sensitive in detecting lipids than biochemical analysis, but both methods—as well as conventional fluorescence microscopy—were comparable in detecting polyphosphates. Discrepancies in the detection of starch by Raman microscopy are discussed. The power of Raman microscopy was proven to be particularly valuable in the detection of guanine, which was traceable by its unique vibrational signature. Guanine microcrystals occurred specifically at around the time of DNA replication and prior to nuclear division. Interestingly, guanine crystals co-localized with polyphosphates in the vicinity of nuclei around the time of nuclear division
LB  - PUB:(DE-HGF)16
C6  - 33401566
UR  - <Go to ISI:>//WOS:000610032700001
DO  - DOI:10.3390/cells10010062
UR  - https://juser.fz-juelich.de/record/904507
ER  -