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@ARTICLE{Moudkov:904507,
      author       = {Moudříková, Šárka and Ivanov, Ivan Nedyalkov and
                      Vítová, Milada and Nedbal, Ladislav and Zachleder, Vilém
                      and Mojzeš, Peter and Bišová, Kateřina},
      title        = {{C}omparing {B}iochemical and {R}aman {M}icroscopy
                      {A}nalyses of {S}tarch, {L}ipids, {P}olyphosphate, and
                      {G}uanine {P}ools during the {C}ell {C}ycle of {D}esmodesmus
                      quadricauda},
      journal      = {Cells},
      volume       = {10},
      number       = {1},
      issn         = {2073-4409},
      address      = {Basel},
      publisher    = {MDPI},
      reportid     = {FZJ-2021-06077},
      pages        = {62 -},
      year         = {2021},
      abstract     = {Photosynthetic energy conversion and the resulting
                      photoautotrophic growth of green algae can only occur in
                      daylight, but DNA replication, nuclear and cellular
                      divisions occur often during the night. With such a
                      light/dark regime, an algal culture becomes synchronized. In
                      this study, using synchronized cultures of the green alga
                      Desmodesmus quadricauda, the dynamics of starch, lipid,
                      polyphosphate, and guanine pools were investigated during
                      the cell cycle by two independent methodologies;
                      conventional biochemical analyzes of cell suspensions and
                      confocal Raman microscopy of single algal cells. Raman
                      microscopy reports not only on mean concentrations, but also
                      on the distribution of pools within cells. This is more
                      sensitive in detecting lipids than biochemical analysis, but
                      both methods—as well as conventional fluorescence
                      microscopy—were comparable in detecting polyphosphates.
                      Discrepancies in the detection of starch by Raman microscopy
                      are discussed. The power of Raman microscopy was proven to
                      be particularly valuable in the detection of guanine, which
                      was traceable by its unique vibrational signature. Guanine
                      microcrystals occurred specifically at around the time of
                      DNA replication and prior to nuclear division.
                      Interestingly, guanine crystals co-localized with
                      polyphosphates in the vicinity of nuclei around the time of
                      nuclear division},
      cin          = {IBG-2},
      ddc          = {570},
      cid          = {I:(DE-Juel1)IBG-2-20101118},
      pnm          = {2172 - Utilization of renewable carbon and energy sources
                      and engineering of ecosystem functions (POF4-217)},
      pid          = {G:(DE-HGF)POF4-2172},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {33401566},
      UT           = {WOS:000610032700001},
      doi          = {10.3390/cells10010062},
      url          = {https://juser.fz-juelich.de/record/904507},
}