TY  - JOUR
AU  - Smolentseva, Anastasia
AU  - Goncharov, Ivan M.
AU  - Yudenko, Anna
AU  - Bogorodskiy, Andrey
AU  - Semenov, Oleg
AU  - Nazarenko, Vera V.
AU  - Borshchevskiy, Valentin
AU  - Fonin, Alexander V.
AU  - Remeeva, Alina
AU  - Jaeger, Karl-Erich
AU  - Krauss, Ulrich
AU  - Gordeliy, Valentin
AU  - Gushchin, Ivan
TI  - Extreme dependence of Chloroflexus aggregans LOV domain thermo- and photostability on the bound flavin species
JO  - Photochemical & photobiological sciences
VL  - 20
IS  - 12
SN  - 1474-905X
CY  - Cambridge
PB  - Royal Society of Chemistry
M1  - FZJ-2022-00320
SP  - 1645 - 1656
PY  - 2021
AB  - Light-oxygen-voltage (LOV) domains are common photosensory modules that found many applications in fluorescence microscopy and optogenetics. Here, we show that the Chloroflexus aggregans LOV domain can bind different flavin species (lumichrome, LC; riboflavin, RF; flavin mononucleotide, FMN; flavin adenine dinucleotide, FAD) during heterologous expression and that its physicochemical properties depend strongly on the nature of the bound flavin. We show that whereas the dissociation constants for different chromophores are similar, the melting temperature of the protein reconstituted with single flavin species varies from ~ 60 °C for LC to ~ 81 °C for FMN, and photobleaching half-times vary almost 100-fold. These observations serve as a caution for future studies of LOV domains in non-native conditions yet raise the possibility of fine-tuning various properties of LOV-based fluorescent probes and optogenetic tools by manipulating the chromophore composition.
LB  - PUB:(DE-HGF)16
C6  - 34796467
UR  - <Go to ISI:>//WOS:000720258200001
DO  - DOI:10.1007/s43630-021-00138-3
UR  - https://juser.fz-juelich.de/record/905009
ER  -