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| Hauptseite > Publikationsdatenbank > Extreme dependence of Chloroflexus aggregans LOV domain thermo- and photostability on the bound flavin species > print |
| Hauptseite > Publikationsdatenbank > Extreme dependence of Chloroflexus aggregans LOV domain thermo- and photostability on the bound flavin species > print |
| 001 | 905009 | ||
| 005 | 20240627202007.0 | ||
| 024 | 7 | _ | |a 10.1007/s43630-021-00138-3 |2 doi |
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| 100 | 1 | _ | |a Smolentseva, Anastasia |0 P:(DE-HGF)0 |b 0 |
| 245 | _ | _ | |a Extreme dependence of Chloroflexus aggregans LOV domain thermo- and photostability on the bound flavin species |
| 260 | _ | _ | |a Cambridge |c 2021 |b Royal Society of Chemistry |
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| 520 | _ | _ | |a Light-oxygen-voltage (LOV) domains are common photosensory modules that found many applications in fluorescence microscopy and optogenetics. Here, we show that the Chloroflexus aggregans LOV domain can bind different flavin species (lumichrome, LC; riboflavin, RF; flavin mononucleotide, FMN; flavin adenine dinucleotide, FAD) during heterologous expression and that its physicochemical properties depend strongly on the nature of the bound flavin. We show that whereas the dissociation constants for different chromophores are similar, the melting temperature of the protein reconstituted with single flavin species varies from ~ 60 °C for LC to ~ 81 °C for FMN, and photobleaching half-times vary almost 100-fold. These observations serve as a caution for future studies of LOV domains in non-native conditions yet raise the possibility of fine-tuning various properties of LOV-based fluorescent probes and optogenetic tools by manipulating the chromophore composition. |
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| 700 | 1 | _ | |a Goncharov, Ivan M. |0 P:(DE-HGF)0 |b 1 |
| 700 | 1 | _ | |a Yudenko, Anna |0 P:(DE-HGF)0 |b 2 |
| 700 | 1 | _ | |a Bogorodskiy, Andrey |0 P:(DE-HGF)0 |b 3 |
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| 700 | 1 | _ | |a Gushchin, Ivan |0 P:(DE-HGF)0 |b 12 |e Corresponding author |
| 773 | _ | _ | |a 10.1007/s43630-021-00138-3 |g Vol. 20, no. 12, p. 1645 - 1656 |0 PERI:(DE-600)2072584-X |n 12 |p 1645 - 1656 |t Photochemical & photobiological sciences |v 20 |y 2021 |x 1474-905X |
| 856 | 4 | _ | |u https://juser.fz-juelich.de/record/905009/files/Autorenfassung_Smolentseva.pdf |y Published on 2021-12-18. Available in OpenAccess from 2022-12-18. |
| 856 | 4 | _ | |u https://juser.fz-juelich.de/record/905009/files/Smolentseva2021_Article_ExtremeDependenceOfChloroflexu.pdf |y Restricted |
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