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@INPROCEEDINGS{Metzner:905565,
      author       = {Metzner, Ralf and Chlubek, Antonia and Bühler, Jonas and
                      Pflugfelder, Daniel and Schurr, Ulrich and Huber, Gregor and
                      Koller, Robert and Jahnke, Siegfried},
      title        = {{C}arbon dynamics in nodulated pea root systems: 3{D}
                      imaging andquantification with short lived isotopes},
      reportid     = {FZJ-2022-00806},
      year         = {2021},
      abstract     = {Biological nitrogen fixation by root nodules of legumes is
                      one of the most important sources for nitrogen in natural
                      ecosystems and low-input agriculture systems. The fact that
                      the host plants of the nodules provide carbon components
                      from photosynthesis in exchange for the nitrogen is long
                      known, as are many details about how the host plants
                      interact with the rhizobia in terms of nodule development.
                      However, there is still a lack in understanding how plants
                      modulate carbon allocation to a nodulated root system as a
                      dynamic response to abiotic stimuli. One reason is that most
                      approaches are based on destructive sampling (e.g. stable
                      isotope tracers), making investigation of localized carbon
                      allocation dynamics in the root system difficult. We
                      employed non-invasive Positron Emission Tomography (PET) to
                      follow the allocation of leaf-supplied 11C tracer towards
                      individual nodules in a three-dimensional (3D) root system
                      of pea (Pisum sativum). Nitrate was applied to the root
                      system to rapidly shut down biological nitrogen fixation and
                      follow the effect on carbon allocation dynamics for the
                      first time at this spatial and temporal resolution. This
                      treatment lead to a reduction of 11C tracer allocation to
                      nodules by $40\%$ - $47\%$ in 5 treated plants within 42h
                      while the change in control plants was less than $11\%.$ Our
                      study demonstrates the strength of using 11C tracers in a
                      PET approach for non-invasive quantification of dynamic
                      carbon allocation in growing plants over several days and
                      within the 3D structure of a root system.},
      month         = {May},
      date          = {2021-05-24},
      organization  = {Rooting 2021 9th International
                       Symposium on Root Development,
                       Nottingham (UK), 24 May 2021 - 28 May
                       2021},
      subtyp        = {After Call},
      cin          = {IBG-2},
      cid          = {I:(DE-Juel1)IBG-2-20101118},
      pnm          = {2171 - Biological and environmental resources for
                      sustainable use (POF4-217)},
      pid          = {G:(DE-HGF)POF4-2171},
      typ          = {PUB:(DE-HGF)24},
      url          = {https://juser.fz-juelich.de/record/905565},
}